斑唇马先蒿的化学成分分离与鉴定  

Purification and Identification of Chemical Constituents of Pedicularis longiflora Rudolph. var. Tubiformis( Klotz). Tsoong

作　　者：邓余[1] 王秋入 王建华[1,3] 唐红梅[1] 李成洪[1] DENG Yu;WANG Qiu-ru;WANG Jian-hua;TANG Hong-mei;LI Cheng-hong(Chongqing Academy of Ani- real Sciences, Chongqing 402460, China;School of Medicine, Shandong University, Jinan 250012, China;School of Biological Engineering, Chongqing University, Chongqing 400044, China)

机构地区：[1]重庆市畜牧科学院畜禽疫病和兽药研究所,重庆402460 [2]山东大学临床医学院,济南250012 [3]重庆大学生物工程学院,重庆400044

出　　处：《中国药学杂志》2017年第24期2146-2150,共5页Chinese Pharmaceutical Journal

基　　金：重庆市基本科研业务费资助(16438);重庆市农业发展资金资助(17412)

摘　　要：目的研究斑唇马先蒿的化学成分及其抗氧化活性。方法采用正相硅胶色谱柱、反相C18硅胶色谱柱、大孔树脂和高效液相等柱色谱分析手段,对藏药斑唇马先蒿的化学成分进行系统的分离纯化,并通过清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基的能力考察了甲醇粗提物、洗脱组分和化合物6~10的抗氧化活性。结果得到10个化合物,根据化合物的波谱数据和理化性质分别将其鉴定为去氧土大黄苷[(E)-3-(β-D-glucopyranosyloxy)-5-hydroxy-4'-methoxystilbene](1)、β-谷甾醇(β-sitosterol)(2)、鸢尾葡萄糖苷(iridotrial glucoside)(3)、木犀草素(luteolin)(4)、苜蓿素(5,7,4'-trihydroxy-3',5'-dimethoxyflavone)(5)、松果菊苷(echinacoside)(6)、焦地黄苯乙醇苷A1(jionoside A1)(7)、甘蓝苷H1(kankanoside H1)(8)、甘蓝苷l(kankanoside l)(9)和马先蒿苷Ⅰ(pedieulariosideⅠ)(10)。另外,甲醇洗脱组分和化合物6的抗氧化活性较好。结论化合物1,3和6~10为首次从该植物中分到。OBJECTIVE To investigate the chemical constituents of Pedicularis longiflora Rudolph. var. tubiformis （Klotz）. Tsoong systematically. METHODS Silica-gel column, reversed phase C18 column, macroporous resin column and Sephadex LH -20 column were employed to purify the chemical constituents. The ability to remove DPPH free radicals was determined to evaluate the an- tioxidant activities of the crude extract, eluted fractions and compounds. RESULTS Ten compounds were obtained and identified as （E） -3- （fl-D-glucopyranosyloxy） -5-hydroxy-4 ＇-methoxystilbene （ 1 ）, fl-sitosterol （ 2 ）, iridotrial glucoside （ 3 ）, luteolin （4）, 5,7,4 ＇- trihydroxy-3＇ ,5＇-dimethoxyflavone （5） , echinacoside （6） , jionoside A1 （7） , kankanoside Hi （ 8 ） , kankanoside 1 （9） and pedieu- larioside I （10） based on the NMR spectra together with physical and chemical properties. In addition, the methanol-eluted fractions and compound 6 showed moderate antioxidant activity. CONCLUSION Compounds 1,3 and 6 - 10 are isolated form Pedicularis lon- giflora Rudolph. var. tubiformis （Klotz）. Tsoong for the first time.

关 键 词：斑唇马先蒿 1 1-二苯基-2-三硝基苯肼(DPPH) 抗氧化活性 松果菊苷 焦地黄苯乙醇苷A1 甘蓝苷Hl 

分 类 号：R284[医药卫生—中药学]