玛咖中总游离氨基酸的提取纯化工艺研究  

Optimization of extraction and purification process of total free amino acids from Maca

作　　者：崔耀天[1] 赵乐荣 于建伟 邹鹏飞 庞遵霆 冯坤苗 韩春超[1] CUI Yao-tian;ZHAO Le-rong;YU Jian-wei;ZOU Peng-fei;PANG Zun-ting;FENG Kun-mao;HAN Chun-chao(Shandong University of Traditional Chinese Medicine, Jinan 250355;Yantai Xinshidai Health Industry Co., Ltd., Yantai 264006)

机构地区：[1]山东中医药大学,济南250355 [2]烟台新时代健康产业日化有限公司,烟台264006

出　　处：《食品科技》2017年第12期235-238,共4页Food Science and Technology

摘　　要：目的:确定玛咖中总游离氨基酸的最佳提取纯化工艺。方法:在单因素试验基础上,设计L9(34)正交试验考察料液比、提取时间、提取次数3个因素对玛咖总游离氨基酸提取率的影响,并通过紫外分光光度法测定玛咖总游离氨基酸含量,将玛咖总游离氨基酸浸出量作为考察指标,从而优化玛咖总游离氨基酸提取工艺。利用732阳离子交换树脂对氨基酸进行纯化,考察阳离子交换树脂动力学吸附和吸附时间、洗脱剂的浓度,确定玛咖氨基酸纯化工艺。结果:玛咖总游离氨基酸最佳提取工艺为乙醇浓度70%、料液比1 g:10 m L、提取时间4 h、提取3次,在此提取工艺下,测得玛咖总游离氨基酸浸出量为39.32 mg/g。玛咖氨基酸纯化工艺为上柱液p H2,上柱后吸附6 h,洗脱液为3%氨水。经过纯化,产物纯度75.29%。结论:玛咖中总游离氨基酸的提取纯化方法稳定、可行。Objective： To determine the optimum extraction and purification process of total free amino acids in Maca. Methods： Basis on single factor tests, designed L9（34） orthogonal test to investigate three factors： solid-liquid ratio, extraction time, extraction times, which effect on the total free amino acids extraction rate. Determined the content of total free amino acids through ultraviolet and visible spectrophotpmetry, and the content of total free amino acids as the marker, optimized the exraction process of total free amino acids. Amino acids was purified by 732 cation exchange resin. studied the dynamic adsorption propreties and adsorption time of cation exchange resin and the concentration of the reagent, to determine the purification process of amino acids in Maca. Results： Optimal extraction conditions of total free amino acids in Maca were as follows： ethanol concentration 70%, solid-liquid ratio 1 g：10 m L, extracting for 4 hours, 4 times, extraction rate of total free amino acids in Maca was 39.32 mg/g. Optimal extraction conditions of amino acids in Maca were as follows： the p H value of the sample was 2, the column was washed by 3% ammonia after 6 h. After purification, the product purity was 75.29%. Concludions： This extraction and purification method is stable and feasible.

关 键 词：玛咖 总游离氨基酸 提取 纯化 

分 类 号：R284.2[医药卫生—中药学]