毛白杨PtoWOX11/12a对杨树扦插苗生长发育的影响  

Effects of PtoWOX11/12a Gene from Populus tomentosa on the Growth and Development of Cutting Seedlings in Poplar

作　　者：李真 王留强 卢孟柱[1] Li Zhen;Wang Liuqiang;Lu Mengzhu(State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091)

机构地区：[1]林木遗传育种国家重点实验室,国家林业局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学》2017年第11期69-76,共8页Scientia Silvae Sinicae

基　　金：“十二五”863计划课题“林木优质、速生性状调控基因的分离及育种技术研究”(2013AA102702)

摘　　要：【目的】WOX转录因子家族是一类植物特有的转录因子家族,在植物胚胎建成、干细胞分裂和分化的维持以及器官的形成过程中发挥重要调控作用。本研究分析毛白杨Pto WOX11/12a基因对转基因84K杨叶形、茎等生长发育的影响,分析毛白杨不定根诱导过程中及过表达和抑制表达Pto WOX11/12a转基因84K杨中Pto WOX11/12a基因、生长素合成基因YUCCA1和YUCCA8的表达模式,为深入研究WOX基因对植物生长发育的调控机制提供参考。【方法】对在温室生长1~3个月的过表达和抑制表达Pto WOX11/12a转基因以及非转基因84K杨(对照)的叶形、株高和地径进行比较;通过组织切片对转基因和非转基因84K杨的第5、9和13节间的解剖学特征进行分析,并对各节间的形成层和木质部宽度进行比较;利用实时荧光定量PCR(q PCR)技术,分析Pto WOX11/12a、生长素合成基因YUCCA1和YUCCA8在毛白杨不定根产生过程中及在过表达和抑制表达Pto WOX11/12a转基因84K杨中的表达模式。【结果】在叶形上,过表达Pto WOX11/12a转基因84K杨叶片长度与非转基因84K杨(对照)没有显著差异,但是宽度显著大于对照;抑制表达Pto WOX11/12a转基因84K杨的叶长和叶宽都明显小于对照,且叶边缘呈现锯齿状缺刻。在植株的株高和地径方面,过表达和抑制表达Pto WOX11/12a转基因84K杨的株高和地径都明显低于对照,且存在显著差异;茎解剖分析发现,过表达Pto WOX11/12a转基因84K杨木质部宽度小于对照,形成层细胞层数多,而抑制表达Pto WOX11/12a转基因84K杨木质部宽度同样小于对照,但与过表达植株相比,木质部宽度相对变大,形成层细胞层数变少。q PCR结果显示Pto WOX11/12a基因在毛白杨不定根产生过程中被诱导并持续表达,而生长素合成基因YUCCA1和YUCCA8在诱导培养3天后表达量才迅速增加,但在过表达Pto WOX11/12a转基因植株中表达量升高,在抑制表达植株中表达量降低�【Objective】 WOX transcription factor family is one of the plant-specific transcription factor families,which have been demonstrated playing an important role in the embryonic patterning,stem cell maintenance and proliferation and lateral organ development. This study analyzed the roles of PtoWOX11/12 a gene from Populus tomentosa in the development of leaves and stems of transgenic 84 K poplar（ Populus alba × P. glandulosa cl. 84 K）. The gene expression level during the process of adventitious roots formation of Populus tomentosa,and in overexpression and suppressed expression of PtoWOX11/12 a transgenic 84 K poplars were analyzed. Our research provides a foundation for further studies of the roles of WOXs gene in plant development.【Method】 The leaf shape,height and basal diameter of one-month-old tothree-month-old transgenic lines and non-transgenic 84 K poplar（ control） in the greenhouse were measured and compared. The anatomy of stem sections of the 5 th,9 thand 13 thinternodes of transgenic lines and control plants was analyzed and the width of xylem and cambium were compared. The expression of PtoWOX11/12 a,YUCCA1 and YUCCA8 during the process of adventitious roots development in P. tomentosa and in overexpression and suppressed expression of PtoWOX11/12 a transgenic 84 K poplars was examined using quantitative real-time PCR（ q PCR）.【Result】 There were no significant differences between overexpression of PtoWOX11/12 a and control plants in leaf length,but the leaf width of overexpression of Pto WOX11/12 a was sharply larger than the control. The leaf length and width of suppression expression PtoWOX11/12 a transgenic poplars were less than control,and the leaf margin had serrate incision. The height and diameter of overexpression and suppression expression PtoWOX11/12 a transgenic poplars were significantly less than the control poplars. The anatomy analysis indicated that the xylem, the number of cambium layers of overexpression PtoWOX11/12 a transgenic poplars was thinner than the

关 键 词：杨树 PtoWOX11/12a 基因表达 实时荧光定量PCR 不定根 形成层 

分 类 号：S718.46[农业科学—林学]