尾吊复合隔离导致的大鼠抑郁样行为研究  

Study on Depression-like Behavior Induced by Tail Suspension Combined with Isolation in Rats

作　　者：张永亮[1] 陈海龙[2] 王婷梅[1] 王艳利[1] 冀国华[2] 吕柯[2] 李莹辉[1,2] 曲丽娜[2] Zhang Yongliang;Chen Hailong;Wang Tingmei;Wang Yanli;Ji Guohua;Lv Ke;Li Yinghui;Qu Lina

机构地区：[1]西北工业大学生命学院,陕西西安710072 [2]中国航天员科研训练中心航天医学基础与应用国家重点实验室,北京100094

出　　处：《航天医学与医学工程》2017年第6期401-405,共5页Space Medicine & Medical Engineering

基　　金：中国航天医学工程预先研究项目(2015SY54A0501);航天医学基础与应用国家重点实验室自主研究项目(SMFA15B01)

摘　　要：目的研究2周的尾吊复合隔离环境所导致的大鼠抑郁样行为。方法 24只雄性SD大鼠随机平均分为3组:对照(Con)、尾吊(tail suspension,TS)、尾吊复合隔离(tail suspension combined with isolation,TS+I)。造模前后检测摄食量和体重变化,造模结束后依次进行空场、新奇事物探索、强迫游泳实验。结果 TS和TS+I组大鼠体重和摄食量低于对照组,且TS+I组最低。TS和TS+I组大鼠的主动性活动显著少于对照组,且TS+I组大鼠最少。TS和TS+I组大鼠探索潜伏期变长,且探究行为减少,其中TS+I组大鼠表现最差。TS和TS+I组大鼠的攀爬次数少于对照组,TS+I组大鼠不动时间最长。结论 2周的尾吊复合隔离环境导致的抑郁样行为比单一尾吊更为严重,此模型可用于航天抑郁发病机制研究及对抗药物开发。Objective To investigate the depression-like behavior induced by tail suspension combined with isolation in rats. Methods Twenty-four male SD rats were randomly divided into 3 groups： control（Con）,tail suspension（TS） and tail suspension combined with isolation（TS ＋ I）. Four typical methods including food intake and bodyweight variation test,open field test,novelty exploration test and forced swimming test were deployed to assess emotional activity after 2-week simulation. Results The body weight and food intake of TS and TS ＋ I group were significantly reduced,and the TS ＋ I group was the lowest group. In the open field test,the locomotor activity of TS and TS ＋ I group were lower than those of Con group,and the locomotor activity of TS ＋ I group was the least. In the novelty exploration test,the latency of TS and TS ＋ I group were longer than those of control group,meanwhile,the rats spent less time on the novelty. Among the three groups,the performance of TS ＋ I group was the worst. In the forced swimming test,the climbing behavior in TS and TS ＋ I group was decreased significantly,the immobility time of TS ＋ I group was longer than those of TS and Con group. Conclusion Compared with tail suspension,tail suspension combined with isolation can lead more serious depression-like behavior in rats. These results could provide an appropriate animal model for the study on pathogenesis of depression and countermeasure drug development under space circumstances.

关 键 词：尾吊 隔离 大鼠 抑郁样行为 

分 类 号：R853[医药卫生—航空、航天与航海医学]