仙灵骨葆胶囊对去势大鼠股骨组织形态计量学和OCN、Cbfα1 mRNA的影响  

Effect of Xianling Gubao capsule on femoral histomorphometry and OCN and Cbfα1 mRNA in ovariectomized rats

作　　者：孙伟珊 霍少川 王晓东[1] 孙平[1] 王海彬 SUN Wei-shan;HUO Shao-chuan;WANG Xiao-dong;et al(First Affiliated Hospital of Guangdong Medical University, Guangzhou 510080, China)

机构地区：[1]广东药科大学附属第一医院,510080 [2]广州中医药大学国家重点学科中医骨伤科重点实验室

出　　处：《中国实用医药》2017年第34期193-196,共4页China Practical Medicine

基　　金：国家自然科学基金(项目编号:81373655);国家自然科学基金资助项目(项目编号:81774339)

摘　　要：目的探讨仙灵骨葆胶囊对去势大鼠股骨形态计量学和成骨相关基因骨钙素(OCN)、核心结合因子(Cbfα1)mRNA的影响。方法 50只6月龄雌性SD大鼠,随机分为A组(作为空白对照)、B组(作为模型)、C组(给予高剂量仙灵骨葆胶囊)、D组(给予低剂量仙灵骨葆胶囊)、E组[给予阿仑膦酸钠维D_3片(福美加)],每组10只。药物干预3个月后,采用X线显微断层显像仪检测左侧离体股骨骨密度(BMD)和骨小梁数量(TbN),逆转录-聚合酶链式反应(RT-PCR)检测右侧股骨组织OCN、Cbfα1 mRNA表达,比较各组检测结果。结果干预3个月后,B组左侧股骨BMD、TbN明显低于A组,差异具有统计学意义(P<0.05);与B组比较,C、E组左侧股骨BMD明显增高,E组左侧股骨TbN明显增高,差异具有统计学意义(P<0.05);与E组比较,C、D组左侧股骨BMD、TbN明显降低,差异具有统计学意义(P<0.05);五组左侧股骨BMD、TbN比较差异具有统计学意义(F=86.400、28.027,P<0.05)。干预3个月后,与A组比较,B组右侧股骨Cbfα1、OCN mRNA表达量明显降低,差异具有统计学意义(P<0.05);与B组比较,C、D、E组右侧股骨Cbfα1 mRNA表达量明显增高(P<0.05),C、E组右侧股骨OCN mRNA表达量明显增高,差异具有统计学意义(P<0.05);与E组比较,C组右侧股骨Cbfα1和OCN mRNA表达量明显增高(P<0.05),D组右侧股骨Cbfα1 mRNA表达量明显降低,差异具有统计学意义(P<0.05);五组大鼠右侧股骨Cbfα1、OCN mRNA比较差异具有统计学意义(F=13.233、48.338,P<0.05)。结论仙灵骨葆胶囊提高去势大鼠股骨BMD可能是通过提高OCN、Cbfα1 mRNA表达量发挥作用,TbN和BMD及成骨基因OCN、Cbfα1之间的关系尚需要进一步研究。Objective To discuss the effect of Xianling Gubao capsule on femoral histomorphometry and osteocalcin（OCN） and core binding factor（Cbfα1） m RNA in ovariectomized rats. Methods A total of 50 6-month-old female SD rats were randomly divided into group A（blank control）, group B（model）, group C（high-dose of Xianling Gubao capsule）, group D（low-dose Xianling Gubao capsule） and group E [alendronate sodium and vitamin D3 tablets（Fumega）], with 10 rats in each group. After 3 months of drug intervention, the left femur bone mineral density（BMD） and trabecular number（TbN） were measured by X-ray microscopy, and the expression of OCN and Cbf α1 mRNA in right femurs was detected by reverse transcription polymerase chain reaction（RT-PCR）. The results of each group were compared. Results After 3 months of drug intervention, group B had obviously lower left femur BMD, TbN than group A, and the difference was statistically significant（P〈0.05）. Compared with group B, group C and E had obviously higher left femur BMD and group E had obviously higher left femur TbN. Their difference was statistically significant（P〈0.05）. Compared with group E, group C and E had obviously lower left femur BMD and TbN, and their difference was statistically significant（P〈0.05）. Five groups had statistically significant difference in left femur BMD and TbN（F=86.400, 28.027, P〈0.05）. After 3 months of drug intervention, group B had obviously lower right femur Cbfα1 and OCN mRNA expression than group A, and the difference was statistically significant（P〈0.05）. Compared with group B, group C, D and E had obviously higher right femur Cbfα1 mRNA expression（P〈0.05）, and group C and E had obviously higher right femur OCN mRNA expression. Their difference was statistically significant（P〈0.05）. Compared with group E, group C had obviously higher right femur Cbfα1 and OCN mRNA expression（P〈0.05）, and group D had obviously lower right femur Cbfα1 mRNA expression. Their d

关 键 词：仙灵骨葆胶囊 去势大鼠 骨质疏松 骨钙素 核心结合因子 

分 类 号：R274.9[医药卫生—中西医结合]