香蕉茎秆水煮液联合西药抗菌药对产CTX-M型超广谱β-内酰胺酶大肠杆菌的抑菌效果研究  

Antibacterial Effect of Banana Straw Water Extracts Combined with Antimicrobials against CTX-M Type Extended Spectrum β-lactamases Producing Escherichia coli

作　　者：王莉贞[1] 宾银燕 罗玲美 李铮 王光娥 司红彬[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005

出　　处：《畜牧与饲料科学》2017年第10期8-10,18,共4页Animal Husbandry and Feed Science

基　　金：国家自然科学基金资助项目(31460675);南宁市科学研究与技术开发计划(20161267)

摘　　要：为了研究香蕉茎秆水煮液联合西药抗菌药对产CTX-M型超广谱β-内酰胺酶大肠杆菌的抑菌效果,应用微量肉汤二倍稀释法测定香蕉茎秆水煮液及阿莫西林、阿米卡星、磷霉素、恩诺沙星、痢菌净、阿奇霉素、利福平、磺胺间甲氧嘧啶钠、头孢噻呋钠等19种西药抗菌药物对产CTX-M型超广谱β-内酰胺酶大肠杆菌的最小抑菌浓度(MICs);采用棋盘法检测香蕉茎秆水煮液与19种西药抗菌药物对产CTX-M型超广谱β-内酰胺酶大肠杆菌联合抑菌作用,通过计算其最小部分抑菌浓度指数(FICI)来判断药物相互作用效应。结果发现,香蕉茎秆水煮液与痢菌净、磷霉素联用时,FICI≤0.5,表现为协同效应;香蕉茎秆水煮液与头孢曲松钠、头孢他啶、头孢噻呋钠、头孢噻肟、阿莫西林、磺胺间甲氧嘧啶钠、阿奇霉素、氟苯尼考及利福平联用时,0.5<FICI≤1,表现为相加效应;香蕉茎秆水煮液与环丙沙星、左旋氧氟沙星及林可霉素联用时,1<FICI≤2,表现为无关效应;香蕉茎秆水煮液与克林沙星及加替沙星联用时,FICI>2,表现为颉颃效应。由该试验结果可以得出,香蕉茎秆水煮液与部分西药抗菌药联合使用能增强其对产CTX-M型超广谱β-内酰胺酶大肠杆菌的抑菌效果。Using CTX-M type extended spectrum 茁- lactamases （ESBLs） producing Escherichia coli （E. coli）, the present study investigated the antibacterial effect of banana straw water extracts combined with ant imicrobial agents in vitro. The minimum inhibitory concentrations （MICs） ofbanana straw waterextracts and the 19 selected antimicrobial agents （amoxicillin, amikacin, fosfomycin, enrofloxacin, mequindox, azithromycin, rifampicin, sulfamonomethoxine sodium, ceftiofur sodium, etc.） against the CTX-M type ESBLs producing E. coli were determined by using broth micro-dilution method; the antibacterial effect of banana straw water extracts combined with each of the 19 selected antimicrobial agents against the CTX-M type ESBLs producing E. coli was assessed with checkerboard micro-dilution method, and the fractional inhibitory concentration index （FICI） was used to evaluate the interactive effect between banana straw water extracts and different antimicrobial agents. The results showed that when banana straw water extracts were used with mequindox or fosfomycin, FICI ≤ 0.5, indicating a synergistic effect; when banana straw water extracts were applied with ceftriaxone sodium, ceftazidime, ceftiofur sodium, cefotaxime, amoxicillin, sulfamonomethoxine sodium, azithromycin, florfenicol, or rifampicin, 0.5 〈FICI 臆 1, indicating an additive effect; when banana straw water extracts were employed with ciprofloxacin, levofloxacin or lincomycin, 1〈FICI ≤ 2, indicating an independent effect; when banana straw water extracts were used with clinafloxacin or gatifloxacin, FICI〉2, indicating an antagonism effect. Our results demonstrate that the banana straw water extracts improve the antibacterial effect of some antimicrobial agents against the CTX-M type ESBLs producing E. coli.

关 键 词：香蕉茎秆水煮液 CTX-M基因 大肠杆菌 抑菌活性 超广谱Β-内酰胺酶 

分 类 号：S853.7[农业科学—临床兽医学] S38[农业科学—兽医学]