结核病血清学检测FolB蛋白(Rv3607c)抗体效果的初步评价  

Assessment of Serological Detecting Effect of FolB Protein(Rv3607c) Antibody in Tuberculosis

作　　者：张康[1] 马国荣 张舒林[1] 邓立[1] 李荣秀[1] Zhang Kang;Ma Guorong;Zhang Shulin;Deng Li;Li Rongxiu(State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University, Shanghai, 20024)

机构地区：[1]上海交通大学,微生物代谢国家重点实验室,上海200240

出　　处：《基因组学与应用生物学》2017年第11期4481-4484,共4页Genomics and Applied Biology

基　　金：国家科技重大专项(2013ZX10003002-005)基金资助

摘　　要：血清学检测是结核病诊断的主要方法之一,Fol B蛋白(Rv3607c)是结核分枝杆菌的一种分泌蛋白,是潜在的血清学检测生物标记物。本研究的目的是评价Fol B蛋白作为生物标记物的特异性和灵敏度。以结核分枝杆菌基因组DNA为模板,通过PCR的方法成功的获得Rv3607c目的基因,克隆到大肠杆菌p ET28a载体上,并在BL21(DE3)中可溶性表达。利用Ni-柱亲和层析系统纯化得到纯度为95%的Fol B蛋白,以此蛋白作为包被抗原,对44例健康人和212例阳性结核患者血清进行了ELISA检测。结果显示Fol B蛋白能区分结核病人和健康对照组,灵敏度和特异性分别为49.3%和90.6%,都比目前公认的临床应用蛋白Pst SI(38 k D)要高,因此Fol B蛋白可以作为一个在结核病临床诊断上有潜在应用意义的生物标记物。Serological detection is one of the prominent diagnosis on tuberculosis, FolB protein （Rv3607c） is a se- creted protein and can be a potential biomarker of serological detection. This study aims to assess the specificity and sensitivity of FolB protein as a biomarker. Taking Mtb genome DNA as complete, we successfully obtain the Rv3607c target gene by PCR and cloning into pET28a vector, the target protein can be expressed soluble in BL21 （DE3）. Purifying the FolB protein through Ni-column affinity chromatography system, the purity is 95%. As the coated antigen, we detect 44 serums of health person and 212 serums of positive tuberculosis by ELISA assay. Re- sult shows that the antibody level can distinguish between two groups, the sensitivity and specificity is 49.3% and 90.6% respectively, and are higher than that of PstSI protein （38 kD） which is currently recognized by clinical appli- cation. Therefore FolB protein can be used as a significant biomarker in the clinical diagnosis of tuberculosis.

关 键 词：结核病 血清学检测 FolB蛋白(Rv3607c) 生物标记物 

分 类 号：R52[医药卫生—内科学]