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作 者:曾令达 谭秋霞 黄建昌[2] 李雪珠 ZENG Lingda;TAN Qiuxia;HUANG Jianchang;LI Xuezhu(School of Life Science, Huizhou University, Huizhou 516007, China;College of Agriculture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China)
机构地区:[1]惠州学院生命科学学院,广东惠州516007 [2]仲恺农业工程学院园艺园林学院,广东广州510225
出 处:《仲恺农业工程学院学报》2017年第4期15-20,共6页Journal of Zhongkai University of Agriculture and Engineering
基 金:广东省科技计划(2013B020304010);惠州市科技计划(2009B010001024);惠州学院自然科学研究计划(C211.0220)资助项目
摘 要:为观察萘乙酸(1-Naphthylacetic acid,NAA)和乙烯利(Ethephon,Eth)对荔枝(Litch chinensis Sonn.)花粉萌发和生长的影响,在基本培养基中分别添加一定质量浓度的NAA和Eth,分别培养糯米糍和桂味2品种荔枝不同批次的花粉,统计花粉萌发率并测定花粉管长度.结果表明:NAA可促进糯米糍和桂味不同批次花粉萌发和花粉管的生长,但其所用适宜浓度不同,其促进第2批花粉萌发的适宜质量浓度为1.0~3.0 mg/L,促进第2批花粉管生长的适宜质量浓度为0.5~3.0 mg/L;较低质量浓度的Eth对2品种荔枝不同批次花粉的萌发和花粉管的生长具有促进作用,其中Eth促进糯米糍、桂味第2批花粉萌发的适宜质量浓度分别为1.0~5.0 mg/L和10.0 mg/L,促进2品种荔枝第2批花粉管生长的适宜质量浓度均为1.0 mg/L;而较高质量浓度(20.0 mg/L)的Eth对2品种荔枝各批次花粉的萌发和花粉管生长具有抑制作用.由此认为,NAA和Eth对糯米糍和桂味2品种荔枝不同批次花粉的萌发和花粉管生长均有促进作用,但其对不同批次花粉起促进作用的适宜质量浓度不同.To observe the effects of 1-Naphthylacetic acid (NAA) and Ethephon (Eth) on germination and growth of litchi (Litch chinensis Sonn. ) pollen, NAA and Eth were added at a certain concentration respectively in basic medium, two varieties different batches of pollen to Nuomici and Guiwei were cul- tured and the pollen germination rate and pollen tube length were determined. The results showed that NAA promoted different batches of pollen germ/nation and pollen tube growth of Nuomici and Guiwei, but the suitable concentration of pollen germination and pollen tube growth in different batches of two varieties of litehi was different. The suitable mass concentration of NAA for second batches of pollen germination was 1.0 - 3.0 mg/L, and that for pollen tube growth was 0.5 - 3.0 mg/L. Low concentration of Eth pro- moted pollen germination and pollen tube growth in different batches of two Litchi euhivars. The appro- priate concentrations of Eth to promote second batch of pollen germination of Nuomici and Guiwei were 1.0-5.0 and 10.0 mg/L, respectively, the suitable concentration of second batch of promoting pollen tube growth of Nuomici and Guiwei was 1.0 mg/L. High concentration (20. 0 mg/L) Eth inhibited the germination and tube growth of different batches of pollen in two Litchi cultivars. The results suggested that NAA and Eth could promote the germination and pollen tube growth of litchi pollen, and the suitable concentration for pollen germination and growth between different batches of pollen was different.
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