透明质酸钠和几丁糖对子宫内膜上皮及成纤维细胞的影响研究  

Study on the effects of hyaluronic acid and chitosan on endometrial epithelium and fibroblasts

作　　者：周建芳 吴晓杰[1] 

机构地区：[1]浙江省嘉兴市妇幼保健院妇科,浙江嘉兴314051

出　　处：《中国现代医生》2017年第34期55-58,共4页China Modern Doctor

基　　金：浙江省嘉兴市科技局科技基金项目(2010KYB108);浙江省嘉兴市科技计划项目(2014AY21032-2)

摘　　要：目的研究几丁糖、透明质酸钠对子宫内膜上皮及成纤维细胞的影响。方法对2014年1月~2015年12月本院收治宫腔粘连患者90例随机分为三组,研究组在宫腔镜粘连分离手术后分别给予几丁糖、透明质酸钠宫腔放置,对照组未作处理,三组患者在术后3个月进行宫腔镜检查及组织活检,分别对术中及术后标本进行镜下子宫内膜上皮及成纤维细胞数目变化进行观察,术后12个月进行宫腔镜检查,对宫腔粘连复发情况进行观察。结果使用几丁糖、透明质酸钠后,宫腔粘连复发率明显低于对照组,宫内组织子宫内膜上皮细胞表达增强,与对照组相比差异有统计学意义(P<0.01),成纤维细胞表达减弱,与对照组相比差异有统计学意义(P<0.05)。结论几丁糖、透明质酸钠能有效预防宫腔粘连的再发,能促进子宫内膜上皮细胞增生,抑制成纤维细胞。Objective To study the effects of chitosan and sodium hyaluronate on＇ endometrial epithelium and fibroblasts. Methods 90 patients with intrauterine adhesion who were admitted to our hospital from January 2014 to December 2015 were randomLy divided into three groups： Study group was given intrauterine placement of chitosan and sodium hyaluronate after intrauterine adhesion resection. The control group was not given any treatment. Three groups of patients were given hysteroscopy and tissue biopsy 3 months after the surgery. The changes of the number of endometrial epithelium and fibroblasts were observed under microscope for intraoperative samples and postoperative samples. Hys teroscopy was performed 12 months after the surgery. The recurrence rate of intrauterine adhesion was observed. Results After the use of chitosan and sodium hyaluronate,the recurrence rate of intrauterine adhesion was significantly lower than that in the control group. The expression of endometrial epithelial cells in intrauterine tissue was enhanced, signif icantly different from that in the control group（P〈0.01）. Fibroblast expression was weakened, and the difference was significant compared with that in the control group（P〈0.05）. Conclusion Chitosan and sodium hyaluronate can effectively prevent the recurrence of intrauterine adhesion and promote the proliferation of endometrial epithelial cells and inhibit fibroblasts.

关 键 词：宫腔粘连 几丁糖 透明质酸钠 子宫内膜上皮细胞 成纤维细胞 

分 类 号：R711.3[医药卫生—妇产科学]