NOD样受体312在幼年特发性关节炎外周血单核细胞中相关性分析  

Analysis on the inflammasomes （NLRP3, NLRP12） expression in peripheral blood mononuclear cells in patients with juvenile idiopathic arthritis

作　　者：谢颖[1] 李洪伟[1] 程苏云 陈挚[1] 李丰[1] 曾萍[1] 曾华松[1] Xie Ying;Li Hongwei;Cheng Suyun;Chen Zhi;Li Feng;Zeng Ping;Zeng Huasong.(Department of Rheumatology and Immunology, Guangzhou Women And Children＇s Medical Center Affiliated Guangzhou Medical University, Guangdong 510623, China)

机构地区：[1]广州医科大学附属广州市妇女儿童医疗中心风湿免疫科,510623

出　　处：《中华风湿病学杂志》2017年第12期795-799,共5页Chinese Journal of Rheumatology

基　　金：广东省自然科学基金（2017A030313557）;广州市卫生局课题（20121A011071）

摘　　要：目的探究炎症小体（NLRP3、NLRPl2）及相关信号蛋白在幼年特发性关节炎（JIA）PBMCs中的表达分析。方法分别采集在广州市妇女儿童医疗中心确诊的55例活动期JIA患儿（病例组：全身型组30例，关节型组25例）；健康对照组22名外周血标本，分离PBMCs及血清，提取并逆转录为cDNA；荧光定量PCR检烈NLRP3、NLRPl2,凋亡相关斑点样蛋白（ASC）、capase．1组间表达差异，计算标准化后的2。An值，ELISA法检测血浆IL-1、IL-4、IL-10表达，组间比较用非参数检验（Kruskal．Wallis检验），NLRP3、NLRPl2与ASC,IL-1、IL4、IL-10，T细胞、B细胞NK细胞绝对计数用Pearson法进行相关性分析。结果NLRP3、NLRPl2、ASC、Capase-1在病例组（全身型组和关节型组）均较对照组高表达（P〈0．05），病例组组间表达水平差异无统计学意义（P〉0．05）。病例组（全身型组、关节型组）IL-1浓度较对照组高（U=1，P=O．001）（U=14，P=O．001）；病例组（全身型组、关节型组）IL．4浓度与对照组差异无统计学意义（U=662，P=0．13）（U：823，P=01535），全身型组IL-10浓度较对照组升高（U=750，P=0．023），而关节型组差异则无统计学意义（U=672，P=0．212）；全身型组与关节型组IL-1（U=658，P=0．408），IL．4（U=475，P=0．068），IL．10（U=475，P=0．195）差异无统计学意义。病例组NLRP3mRNA相对表达量与aSC（r=0．44，P=0．0134）、IL．1（r=0．58，P=0．001）、ESR（r=0．415，P=0．039）、CRP（r=0．438，P=0．046）呈正相关，病例组NLRPl2tuRNA相对表达量与ASC（r=0．5837，P=0．007）、CRP（r=0．46，P=0．0316）、ESR（r=0．003，P=0．56）、CD8＋T（r=0．414，P=0．036）呈正相关。结论JIAPBMCs炎症小体（NLRP3、NLRPl2）异常表达可能与幼年特发性关节炎急性炎症有关，炎症小体或许能成为JIA新的治疗靶标。Objective To explore the expression of inflammasomes （NLRP3, NLRP12） and related signal proteins in the peripheral blood mononuclear ceils （PBMCs） of patients with juvenile idiopathic arthritis （JIA）. Methods Samples of children with definite diagnosis of active JIA in Guangzhou Women and Childrens＂ Medical Center were collected retrospectively. Fifty-five cases were included, among whom 30 were systemic type and 25 were joint type. Blood samples of 22 healthy controls were collected at the same time. Peripheral blood single nuclear cell （PBMCs） were separated and DNA were extracted and reverse transcription （RT） to cDNA. Fluorescent quantitative polymerase chain reaction （PCR） was used to detect NLRP3, NLRP12, ASC, and capase-1 in groups and the difference in their expression between groups were analyzed. Enzyme linked immunosorbent assay （ELISA） was utilized to test plasma levels of interleukin （IL）-6 IL-1, IL-4, IL-10, and their correlation were analyzed. Results The expression of NLRP3, NLRP12, ASC, Capase-1 in the case group （general-group and joint-group） were higher than those in the control group （P〈0.05）, but there was no significant difference in the expression levels between groups （P〉0.05）. The IL-1 concentration of the case group （body-type group, joint-group） was higher tban the control group （P=0.001, U=l） （P=0.001, U=I4）, however, the level of IL-4 of the case group （body-type group, joint-group） was not significantly different from the control group （U=662, P=0.13） （U=823, P=0.535）, IL-10 of the systemic group was higher than that of the control group （U=750, P=0.023）, while there was no difference between groups （U=672, P=0.212）. There were no significant difference in the levels of IL-1 （U=658, P=0.408）, IL-4 （U=475, P=0.068）, IL-10 （U=475, P=0.t95） between groups. The NLRP3 mRNA relative expression levels of the ease group and the ASC （r=0.44, P=-0.013 4） was significant, in addition, IL-1 （P=0.

关 键 词：炎症 关节炎 幼年型类风湿 单核细胞 

分 类 号：R725.9[医药卫生—儿科]