白塞病血循环内皮微粒的检测及临床意义分析  

The role of endothelial microparticles in Behcet＇s disease

作　　者：范秋玉 许珂[1] 张莉芸[1] 张改连[1] 马丹[1] 刘樱 乔鹏燕[1] 李娟[1] 侯睿宏[1] Fan qiuyu;Xu Ke;Zhang Liyun;Zhang Gailian;Ma Dan;Liu Yin;Qiao Pengyan;Li Juan;Hou Ruihong.(Department of Rheumatology and Immunology, Shanxi Medical Sciences Shanxi Da Yi Hospital, Taiyuan 030032, Chin)

机构地区：[1]山西医学科学院山西大医院风湿免疫科,太原030032

出　　处：《中华风湿病学杂志》2017年第12期824-828,I0001,共6页Chinese Journal of Rheumatology

摘　　要：目的内皮微粒不仅可以反映内皮细胞的激活或损伤状态，而且可以调节炎症反应、凝血和血管功能。本研究主要探讨内皮微粒是否可以评估白塞病系统受累情况及作为疾病活动度的新指标。方法本研究共纳入39例白塞病患者，37例高血压病患者和30名性别和年龄相匹配的健康者。采用流式细胞仪检测血浆中CD31~CD42b？内皮微粒水平。各组计量资料以互±s表示，组间比较用t检验，方差分析，采用Spearman／Pearson进行相关分析，以P〈0．05为差异有统计学意义。结果白塞病患者血浆内皮微粒水平[（7．62±0．59）％]高于高血压组[（6．19±1．28）％]和健康对照组[（1．28±0．23）％]（F=6．845，P〈0．05），且与疾病活动度评分呈正相关（r=0．802，P〈O．05）。有血管和消化系统受累白塞病患者血浆内皮微粒水平较无血管和消化道受累患者升高（t=4．707，2．673；P〈0．05）。结论白塞病患者血浆内皮微粒升高，且与疾病活动度相关，提示其可能成为预测白塞病疾病活动度新的指标；内皮微粒水平升高，提示血管、消化系统受累风险增加。Objective Endothelial microparticles （EMPs） are direct indicator of endotheiial cell activation or apoptosis, and may also reflect endothelial inflammation, increased coagulation, and vascular tone. The aim of this study is to investigate whether EMPs would be able to evaluate systemic involvement and be a new indicator of disease activity in Behcet＇s disease （BD）. Methods Thirty-nine consecutive BD patients （who fulfilled the modified International Study Group on BD in 1990 or International Criteria for BD in 2006） and 67 age and sex-matched healthy controls were enrolled （Including 37 patients with hypertension and 30 healthy subjects）. The plasma levels of EMPs were measured by flow cytometry utilizing specific labels for endothelial MPs （CD31＋ and CD42b-）. The measurement data of each group were expressed as x±s, and the comparison data betwen groups were analyzed by independent sample t test and analysis of variance, Spearman/Pearson correlation analysis, P〈0.05 was statistically significant. Results The levels of circulating EMPs （CD31＋ and CD42b-） were significantly elevated in the case group compared with the healthy control group and hypertension （F=6.845, P〈0.05）. Moreover, BD patients plasma EMPs were positively correlated with active BD （r=0.802, P〈0.05）. Vascular involvement in BD patients was higher than in patients without vascular EMPs, t=4.707, P〈O.05. Gastrointestinal involvement in BD patients was more frequent than that in patients without Gastrointestinal involvement, t=2.673, P〈O.05. Conclusion Levels of circulating EMPs are elevated, in BD patients and correlated with disease activity in BD. Elevated EMPs may be a potential indicator to predict disease activity of BD. The plasma level of EMPs is increased, which indicats increased risk of vascular and digestive tract involvement in BD.

关 键 词：贝赫切特综合征 内皮微粒 内皮功能损伤 高血压病 疾病活动度 

分 类 号：R597.9[医药卫生—内科学]