Lactobacillus casei Zhang对扑热息痛诱导的小鼠急性肝损伤的保护作用  

Protective effect of Lactobacillus casei Zhang against Acetaminophen-induced acute liver injury in mice

作　　者：张晓慧[1] 颜妍 赵世敏[1] 王昕旭 刘春妍 谢基明 王玉珍[1] 武春燕[1] ZHANG Xiao-Hui;YAN Yan;ZHAO Shi-Min;WANG Xin-Xu;LIU Chun-Yan;XIE Ji-Ming;WA NG Yu-Zhen;WU Chun-Yan(College of Life Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018 [2]内蒙古自治区人民医院检验科,呼和浩特010020

出　　处：《中国免疫学杂志》2017年第12期1761-1764,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(81260662;81560677);内蒙古杰出青年培育基金(2016JQ08);内蒙古自然科学基金(2016MS0836;2015MS0884)

摘　　要：目的:研究益生菌Lactobacillus casei Zhang(Lcz)对扑热息痛(APAP)所致小鼠急性肝损伤的保护作用及其机制。方法:C57BL/6小鼠被随机分为空白组(Ctrl)、APAP诱导急性肝损伤模型组(Acetaminophen,APAP)、阳性药物组(NAcetylcysteine,NAC)、Lcz预防组(Lcz/APAP)和Lcz对照组(Lcz)。Lcz(1×10~9CFU/ml)连续灌胃30 d后,NAC组在APAP处理前1 h腹腔注射NAC(150 mg/kg)。APAP、NAC以及Lcz/APAP组均腹腔注射APAP(300 mg/kg)。APAP作用18 h后,采集血液和收集肝脏组织,检测血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)的水平。通过Western blot检测肝脏组织中血红素氧化酶(HO-1)、超氧化物歧化酶2(SOD2)、Bcl-2以及TLR4的表达水平。结果:Lcz能抑制APAP诱导的急性肝损伤血清中ALT和AST水平。Lcz提高了HO-1、SOD2和Bcl-2的蛋白表达水平,而降低了APAP诱导的TLR4的表达。结论:益生菌Lcz对APAP诱导的小鼠急性肝损伤有保护作用,其保肝作用机制可能与其抗氧化和抗炎活性有关。Objective：To investigate the protective effects of Lactobacillus casei Zhang （ Lcz） on mice with Acetaminophen （APAP）-induced acute liver injury.Methods：Mice were randomly divided into five groups：Control group （ctrl）,APAP-induced liver injury group （ APAP ） , N-Acetylcysteine positive control group （ NAC ） , Lcz preventive group （ Lcz/APAP ） and Lcz control group （Lcz）.Mice were orally administrated with Lcz （1×109 CFU/ml） for 30 continuous days prior to APAP exposure.Positive control group were intraperitoneally injected with 150 mg/kg NAC 1 h before APAP challenge.Mice in the APAP,NAC and Lcz/APAP group were intraperitoneally injected with 300 mg/kg APAP.Blood and liver samples were collected 18 h post APAP challenge.The expression of HO-1,SOD2,Bcl-2 and TLR4 were detected by Western blot.Results： Lcz could significantly inhibit APAP-induced increase in ALT and AST levels.Furthermore,pretreatment with Lcz increased the hepatic expression of HO-1,SOD2 and Bcl-2,while suppressing the expression of Toll-like receptor 4 （TLR4）.Conclusion：Lcz can protect against APAP-induced acute liver injury in mice.The hepato-protective effects of Lcz are associated with its anti-oxidative and anti-inflammatory capacity.

关 键 词：LactobacilluscaseiZhang 扑热息痛 超氧化物歧化酶2 血红素氧化酶 BCL-2 

分 类 号：R392[医药卫生—免疫学]