葛根素对妊娠期糖尿病大鼠胰腺组织let-7f及免疫因子IL-17、IL-23表达的影响  

Influence of puerarin on pancreatic tissue let-7f and immune factors IL-17 and IL-23 expression of rats with gestational diabetes mellitus

作　　者：刘奇云[1] 马敬[1] 陶艳萍[1] 敬晓涛[1] LIU Qi-Yun;MA Jing;TAO Yan-Ping;JING Xiao-Tao.(First Affiliated Hospital of Kunming Medical University, Kunming 650000, Chin)

机构地区：[1]昆明医科大学第一附属医院,昆明650000

出　　处：《中国免疫学杂志》2017年第12期1843-1848,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金地区项目(81460240);云南省科技厅-昆明医科大学应用基础研究联合专项资金项目青年博士项目[2017FE467(-140)];云南省卫生和计划生育委员会医学后备人才培养计划(H-201646)

摘　　要：目的:探究葛根素对妊娠期糖尿病大鼠胰腺组织let-7f及免疫因子IL-17、IL-23表达的影响。方法:将120只大鼠分为正常对照组、空白对照组、辛伐他汀组、葛根素低剂量组、葛根素中剂量组、葛根素高剂量组,正常对照组以基础饲料、其他组以高脂饲料饲喂2周。辛伐他汀组给予2 mg/kg的辛伐他汀,葛根素组按低、中、高剂量分别给予70 mg/kg、120mg/kg、170 mg/kg葛根素,正常对照组和空白对照组给予等量生理盐水,治疗2周后,测定6组大鼠血糖和血脂水平、胰腺组织中let-7f的表达水平以及血清和胰腺组织中IL-17和IL-23的表达情况。结果:治疗后,辛伐他汀组和葛根素组大鼠的FBG、TC、TG、FFA显著低于空白对照组,且随着葛根素剂量的增加,FBG、TC、TG、FFA的水平显著降低(P<0.05),呈剂量依赖性;辛伐他汀组和葛根素组胰腺组织中let-7f的表达显著高于空白对照组(P<0.05),且呈剂量依赖性;辛伐他汀组和葛根素组血清和胰腺组织中IL-17和IL-23的表达显著高于空白对照组(P<0.05),且呈剂量依赖性。结论:葛根素能提高妊娠期糖尿病大鼠胰腺组织let-7f表达,同时降低血清和胰腺组织中IL-17、IL-23表达,为妊娠期糖尿病临床治疗提供新的思路和理论依据。Objective： To explore the influence of puerarin on pancreatic tissue let-7 f and immune factors IL-17 and IL-23 expression of rats with gestational diabetes mellitus. Methods： 120 cases of rats were divided into the normal control group,the blank control group,simvastatin group,puerarin low-dose group,puerarin medium-dose group and puerarin high-dose group. The normal control group were given basal diet,and the other groups were given high fat diet,all fed for two weeks. The simvastatin group were given2 mg/kg simvastatin,and the puerarin group were given 70 mg/kg,120 mg/kg,170 mg/kg puerarin according to low,medium,high dose,and the normal control group and blank control group were given the same amount of normal saline,after 2 weeks of treatment,blood sugar and blood lipid and let-7 f expression in pancreatic tissue of rats were tested in the six groups. Results： After treatment,FBG,TC,TG and FFA of rats in the simvastatin group and puerarin group were significantly lower than those in the blank control group,and with the increasing of puerarin dose,the levels decreased significantly（ P〈0. 05）,in a dose-dependent manner. Let-7 f expression in pancreatic tissue of the simvastatin group and puerarin group was significantly higher than that in the blank control group（ P〈0. 05）,in a dose-dependent manner. IL-17 and IL-23 expression in serum and pancreatic tissue of the simvastatin group and puerarin group was significantly higher than that of the blank control group（ P〈0. 05）,in a dose-dependent manner. Conclusion： Puerarin can increase let-7 f expression in pancreatic tissue of gestational diabetes mellitus rats with gestational diabetes mellitus,and can reduce IL-17 and IL-23 expression in serum and pancreatic tissue. It can provide reference for clinical treatment of gestational diabetes mellitus.

关 键 词：妊娠期糖尿病 葛根素 let-7f 白细胞介素17 白细胞介素23 

分 类 号：R714.256[医药卫生—妇产科学]