高效液相色谱法同时测定大黄中14种成分的含量  

Simultaneous content determination of 14 components in Rhei Radix et Rhizoma by high performance liquid chromatography method

作　　者：刘月红[1] 黄政海 董玲 裴纹萱 孙裕 高晓燕[1] 

机构地区：[1]北京中医药大学中药学院,北京102488 [2]北京中医药大学生命科学学院,北京100029 [3]兰州佛慈制药股份有限公司,甘肃兰州730046

出　　处：《中国中药杂志》2017年第23期4514-4519,共6页China Journal of Chinese Materia Medica

基　　金：国家中药标准化项目(ZYBZH-Y-GS-10-B)

摘　　要：建立高效液相色谱法同时测定大黄中大黄素、大黄酚、大黄酸、芦荟大黄素、大黄素甲醚、番泻苷A、番泻苷B、没食子酸、儿茶素、(-)-表儿茶素-3-没食子酸酯、异莲花掌苷、4-4'-羟基苯基-2-丁酮、莲花掌苷、4'-羟基苯基-2-丁酮-4'-O-β-D-(2″-O-桂皮酰基-6″-O-没食子酰基)-葡萄糖苷14个成分含量的方法。采用Agilent Zorbax SB-C18色谱柱(4.6 mm×150 mm,5μm),采用0.05%的磷酸水溶液(A)-乙腈(B)为流动相,梯度洗脱,流速1.0 m L·min-1,柱温40℃,检测波长268 nm。结果表明在线性范围内14个成分线性良好(r>0.999 9);日内精密度和日间精密度均小于3.1%;平均回收率在91.80%~104.1%。同时,对收集到的10个掌叶大黄和10个唐古特大黄合格样品进行定量测定,发现掌叶大黄中含量比较高的成分是芦荟大黄素,唐古特大黄中含量比较高的是4-4'-羟基苯基-2-丁酮,各样品中所有化合物的含量差异都比较大。所建立的含量测定方法能同时测定大黄中14个成分的含量,为大黄药材多成分含量测定和质量控制提供一种简便的方法。To establish an HPLC （high performance liquid chromatography） method for the simultaneous content determination of gallic acid, （＋）-catechin, （-）-epicatechin-3-O-gallate, isolindleyin, 4-（4＇-hydroxyphenyl）-2-hutanone, emodin, chxTsophanol, physcion, aloe-emodin, rhein, lindleyin, 4-（ 4＇-hydroxyphenyl ） -2-butanone-4＇-O--D-（ 2＂-O-galloyl-6＂-O-cinnamoyl ） -glucopyrano- side, seunoside A and sennoside B in Rhei Radix et Rhizoma. The analysis was performed on Agilent Zorbax SB-CI8 （4. 6 mm 150 mm, 5 μm） with 0. 05% phosphoric acid solution （A） - acetonitrile （B） as mobile phase for gradient elution. The flow rate was 1 mL min- 1, with column temperature of 40℃ and the wavelength was set at 268 nm. All calibration curves showed good linearity （r 〉 0. 999 9 ） within the concentration range. Both the intra- and inter-day precision for 14 analytes was less than 3. 1% , with the mean re- covery at the range of 91.80%-104. 1%. Meanwhile, quantitative determination was carried out for 10 qualified samples from Rheum palmatum and 10 qualified samples from R. tanguticum, respectively. It was found that the content of 4-（4＇-hydroxyphenyl） -2-buta- none and aloe-emodin were higher in the R. tanguticum and R. palmatum, respectively, and the content of all the compounds was dif- ferent in each sample. The established HPLC method for simuhaneous content determination of 14 compounds from Rhei Radix et Rhi- zoma could be used for quantitative assessment and quality control of Rhei Radix et Rhizoma.

关 键 词：大黄 高效液相色谱法 蒽醌类 苯丁酮苷类 鞣质类 含量测定 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]