茜草炭不同极性部位化学成分组成及保护氧化损伤活性比较  

Protective effect of different polar fractions of Carbonized Rubiae Radix et Rhizoma(cRRR) against oxidative injury

作　　者：肖林焱 王海丽[1] 陈毅[1] 张丽[1] 程芳芳[1] 单鸣秋[1] 丁安伟[1] 

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023

出　　处：《中国中药杂志》2017年第23期4604-4610,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81473348);江苏省高校优势学科建设工程项目(ysxk-2014);江苏高校品牌专业建设工程项目(PPZY2015A070)

摘　　要：采用氧化低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮细胞(HUVECs)损伤模型,MTT比色法测定细胞活力,评价茜草炭不同极性部位提取物的抗氧化损伤活性,同时采用超高效液相色谱与串联四级杆飞行时间质谱联用技术(UPLC-QTOF-MS)对各极性部位化学成分进行定性研究。结果显示,乙酸乙酯部位与正丁醇部位均可显著提高细胞活力(P<0.01),石油醚部位对细胞活力影响不大,水提取部位对损伤的HUVECs有一定的抑制作用。UPLC-Q-TOF-MS分析结果显示,从茜草炭4个极性部位提取物中共鉴定化合物32种,包括31种醌类及其糖苷类和1个烯萜(茜草哌唑嗪C),石油醚部位、乙酸乙酯部位、正丁醇部位、水提取部位分别鉴定出化合物23,32,26,15种。关联体外细胞实验结果表明,乙酸乙酯部位和正丁醇部位为茜草炭抑制ox-LDL诱导HUVECs损伤的有效部位,为阐明茜草炭的药效物质基础提供科学依据,为茜草炭功效-物质相关性研究奠定基础。The protective effect of different polar fractions of Carbonized Rubiae Radix et Rhizoma （cRRR） against ox-LDL-induced damage to human umbilical vein endothelial cells （HUVECs） was investigated by MTT assay, and the components were identified by using UPLC-Q-TOF-MS. According to the study, ethyl acetate extract and n-butanol extract could increase cell viability （P 〈 0. 01 ）, while petroleum ether extract had no influence, and water extract could even inhibit the cell viability to some degree. Moreover, 32 compounds in four polar fractions were analyzed, including 31 quinones and their glycosides, and one rubiprasins C. Petroleum ether extract, ethyl acetate extract, n-butanol extract and water extract contained 23, 32, 26, 15 compounds, respectively. According to cell experiments in vitro, active fractions were ethyl acetate extract and n-butanol extract. The results could provide scientific references tor further studies on effective material basic of cRRR, and lay a foundation for studies on the relationship between efficacies and materials.

关 键 词：茜草炭 极性部位 化学成分定性 细胞损伤 OX-LDL 

分 类 号：R284[医药卫生—中药学]