人工打洞沉香中1个新的倍半萜  

A new sesquiterpene from Chinese agarwood induced by artificial holing

作　　者：邝彤东 陈惠琴[2,3] 李薇 杨锦玲[2,3] 周丽曼 蔡彩虹[2,3] 董文化 梅文莉[2,3] 戴好富 

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101 [3]海南省沉香工程技术研究中心,海南海口571101

出　　处：《中国中药杂志》2017年第23期4618-4623,共6页China Journal of Chinese Materia Medica

基　　金：海南省自然科学基金创新研究团队项目(2017CXTD020);海南省重大科技计划项目(ZDKJ2016004);海南省重点研发计划项目(ZDYF2016140);海南省重点研发计划项目(ZDYF2017046)

摘　　要：运用多种色谱技术,对人工打洞沉香乙醇提取物的正丁醇相进行化学成分分离,并根据波谱数据结合理化性质共鉴定了7个单体化合物的结构,分别为selina-3,11-dien-9,15-diol(1)、aquilarone D(2)、5α,6β,7α,8β-tetrahydroxy-2-[2-(2-hydroxyphenyl)ethyl]-5,6,7,8-tetrahydrochromone(3)、6,7-dimethoxy-2-[2-(4-methoxyphenyl)ethyl]chromone(4)、syringin(5)、顺式对羟基肉桂酸甲酯(6)和4'-甲氧基肉桂酸(7),其中化合物1为新的倍半萜类化合物,化合物5~7为首次从沉香中分离得到。生物活性测试结果表明化合物6,7对全齿复活线虫具有较好的致死活性,化合物4,6,7对人肝癌细胞株BEL-7402、人胃癌细胞株SGC-7901和人肺癌细胞株A549的生长有不同程度的抑制作用。In order to study the chemical constituents of n-butanol fraction of ethanol extract from Chinese agarwood induced by arti- ficial holing, various chromatographic techniques were carried out to isolate compounds, and the structures of compounds were deter- mined through a combined analysis of physicochemical properties and spectroscopic evidence. Seven compounds were obtained and i- dentified as selina-3,11 -dien-9,15-diol （ 1 ）, aquilarone D （ 2 ）, 5a, 6/3,7 a, 8/3-tetrahydroxy-2 - [ 2 - （ 2 -hydroxyphenyl ） ethyl ] -5,6,7,8- tetrahydrochromone （3）, 6,7-dimethoxy-2 - [ 2- （4 -methoxyphenyl） ethyl~ chromone （ 4 ）, syringin （ 5 ）, methyl （Z） -p -coumarate （ 6 ）, and 4＇-methoxycinnamic acid （7） , among which compound 1 was a new compound and compounds 5-7 were isolated from agarwood for the first time. The bioactivity assay results concluded that compounds 6 and 7 showed certain nematicidal activity against PanagreUusredivivus, and compounds 4, tl and 7 exhibited cytotoxicity against BEL-7402, SGC-7901 and A549 carcinoma cell lines.

关 键 词：沉香 正丁醇相 倍半萜 生物活性 

分 类 号：R284.1[医药卫生—中药学]