不同尿沉渣检测方法在检测尿红细胞形态及参数中的价值分析  

Analysis of the Value of Different Urinary Sediment Detection Methods for Red Cell Morphology and Parameters

作　　者：李祥坤[1] LI Xiang-kun(Department of Laboratory, Suining Central Hospital of Sichuan Province, Suining 629000, Chin)

机构地区：[1]四川省遂宁市中心医院检验科,四川遂宁629000

出　　处：《标记免疫分析与临床》2017年第12期1419-1422,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探究全自动尿沉渣分析仪联合尿干化学分析法在尿沉渣检测中的价值。方法随机选取118份从住院患者处收集的血尿样本,根据临床诊断结果将其分为肾小球疾病组(肾小球性血尿组,n=72)及非肾小球疾病组(非肾小球性血尿组,n=46)。比较两组血尿样本尿平均红细胞体积(MCV)、尿平均红细胞血红蛋白(MCH)水平差异。每份尿样均分为2管(15m L/管),分别予以显微镜人工镜检法及全自动尿沉渣分析仪+尿干化学检测法检测(尿液收集后2h内完成)。记录两种检测方法的尿红细胞(RBC)阳性检出率;以临床诊断结果为依据评估其尿RBC形态参数测定结果差异。结果肾小球性血尿组患者尿MCV、尿MCH水平均明显低于非肾小球性血尿组患者,差异有统计学意义(P<0.05)。118份血尿样本经全自动尿沉渣分析仪+尿干化学检测法测得尿RBC阳性率为76.3%(90/118),显微镜人工镜检法测得尿RBC阳性率75.4%(89/118);两组尿RBC阳性检出率比较,差异无统计学意义(P>0.05)。显微镜人工镜检法鉴别灵敏度为97.2%(70/72)、特异性为84.8%(39/46);全自动尿沉渣分析仪+尿干化学检测法鉴别灵敏度为94.4%(68/72)、特异性为80.4%(37/46),两种方法灵敏度及特异性比较,差异无统计学意义(P均>0.05)。结论全自动尿沉渣分析仪+尿干化学检测法对血尿来源的定位诊断准确性较高且操作性强,临床可将其作为一种可行性途径,为后续治疗工作的顺利开展提供依据。Objective To explore the value of automatic urinary sediment analyzer combined with dry urinalysis in detecting urinary sediment. Methods A total of 118 hematuria samples were randomly collected from inpatients. According to the diagnostic results, they were divided into the glomerular disease group （glomerular hematuria group, n=72） and the non-glomerular disease group （non-glomerular hematuria, n=46）. The urine mean corpuscular volume （MCV） and mean corpuscular hemoglobin （MCH） were compared between the two groups, and each sample was divided into 2 tubes （15ml/ tube）. The two tubes were respectively detected by microscopic manual examination and automatic urinary sediment analyzer combined with dry urinalysis （completed within 2h after collecting urine）. The positive detection rates of urine red blood cells （RBC） by the two methods were recorded. Based on the results of clinical diagnosis, the differences in urine red cell morphology and parameters were evaluated. Results The levels of urine MCV and urine MCH in glomerular hematuria group were significantly lower than those in non-glomerular hematuria group （P〈 0.05）. The positive rates of urine RBC in 118 samples detected by automatic urinary sediment analyzer and dry urinalysis were 76.3% （90/118） and 75.4% （89/118）, respectively （P〉 0.05）. Clinical diagnosis showed that there were 72 samples of renal hematuria and 46 samples of non-renal hematuria among the 118 samples. The sensitivity and specificity of automatic urinary sediment analyzer and dry urinalysis were 97.2% （70/72） and 84.8% （39/46） respectively while those of automatic urinary sediment analyzer were 94.4% （68/72） and 80.4% （37/46）, respectively （P 〉 0.05）. Conclusion The accuracy of automatic urinary sediment analyzer and dry urinalysis is high in the localization diagnosis of hematuria and operability is high. It can be used as a feasible way, providing the basis for the smooth implementation of follow-up work.

关 键 词：显微镜法 全自动尿沉渣分析仪 尿干化学检测法 尿沉渣检查 尿红细胞形态 价值 

分 类 号：R446.12[医药卫生—诊断学]