一株野生侧耳属菌株的鉴定及生物学特性  

Identification and Biological Characteristics of a Wild Pleurotus sp.

作　　者：宋冰[1] 付永平[1] 郭昱秀 李长田[1] 张志武 李玉[1] SONG Bing;FU Yongping;GUO Yuxiu;LI Changtian;ZHANG Zhiwu;LI Yu(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University,Changehun,Jilin 130118;Department of Crop and Soil Sciences,Washington State University,Washington, USA 99164)

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118 [2]华盛顿州立大学作物和土壤科学系,美国华盛顿99164

出　　处：《北方园艺》2017年第24期182-188,共7页Northern Horticulture

基　　金：公益性行业(农业)科研专项资助项目(201503137);国家重点研发计划资助项目(2017YFD0601002);高等学校学科创新引智计划资助项目(D17014);国家重点基础研究发展计划(973)资助项目(2014CB138305);吉林省秸秆综合利用技术创新平台资助项目(吉高平合字[2014]C-1);长春市科技局资助项目(15SS11)

摘　　要：以1株野生侧耳属菌株为试材,采用ITS序列克隆和分析,并结合传统形态学研究对其进行分类鉴定,通过单因素试验确定该菌株的生物学特性。结果表明:该野生菌株为紫孢侧耳(Pleurotus sapidus),其菌丝生长的最佳碳源为玉米粉,最佳氮源为牛肉膏,最适培养温度为30℃,最适pH 7.0。该研究为加快紫孢侧耳的推广和产业化提供了基础和种质资源。A wild fungi stain was used as test material,the strain was identified by molecular identification with ITS sequence cloning and analyzing and combine with the traditional morphological.And the biological characteristics were studied by single factor test.The results showed that the wild strain was identified as Pleurotus sapidus by morphological and ITS analysis.The optimum carbon source was maize meal,the optimum nitrogen source was beef extract,the optimal cultivation temperature was 30 ℃,and the optimal pH was 7.0.This research promoted the basis and genetic resources to increase the market promotion and industrialization development of Pleurotus sapidus.

关 键 词：紫孢侧耳 形态学 ITS 生物学特性 

分 类 号：S646.14[农业科学—蔬菜学]