不同病程尖锐湿疣患者HPV感染和淋巴细胞免疫功能分析  

Analysis of human papilloma virus genotypes and cellular immune function in different courses of patients with condyloma acuminata

作　　者：梁艳华[1] 毕超[1] 梁景耀[1] 杨日东[1] 王焕丽[1] 邓斌[1] 宋卫忠[1] 

机构地区：[1]广州市皮肤病防治所,广州510095

出　　处：《中国艾滋病性病》2017年第12期1138-1140,共3页Chinese Journal of Aids & STD

摘　　要：目的分析不同病程尖锐湿疣(CA)患者感染人乳头瘤病毒(HPV)亚型情况和细胞免疫功能。方法收集CA患者468例,设立病程<3个月的258例患者为A组,>3个月的210例患者为B组,同时设立对照组30例。CA患者取疣体细胞进行HPV-脱氧核糖核酸(DNA)分型检测,抽取静脉血检测淋巴细胞亚群和艾滋病病毒抗体。对照组取泌尿生殖系统脱落细胞,并抽取静脉血,检测同CA患者。结果 468例CA患者中,HPV亚型检出率由高到低依次HPV6(34.2%),HPV11(26.5%),HPV52(12.0%)、HPV16(10.3%)。A、B两组患者共检出HPV亚型阳性结果为754个,低危型321个,占42.6%,高危型433个,占57.4%。A组364个,其中低危型180个(49.5%),高危型184个(50.5%);B组390个,其中低危型141个(36.2%),高危型249个(63.8%)。B组患者多重感染比例为43.8%(92/210),A组为27.1%(70/258),差异有统计学意义(χ2=14.23,P<0.01)。CA患者CD4+、CD4+/CD8+低于对照组,CD8+高于对照组,其中B组显示的差异更为显著。结论 HPV高危亚型感染以及与高危亚型合并的多重感染,同时机体细胞免疫功能低下是CA复发的重要因素。Objective To analyze the subtype distribution of human papilloma virus（HPV）and the expression of lymphocyte subsets in different courses of patients with condyloma acuminate（CA）.Methods A total of 258 patients with a course of less than 3 months were group A and those of 210 patients with more than 3 months were group B.PCR method was used to detect HPV subtypes in warts before treatment.Blood were taken after treatment for 3 months,to analyze subtypes of lymphocytes by flow cytometry,and the results were compared with a normal control group.Results The overall prevalence of HPV in patients with CA was 100% with 19 genotypes.The main HPV genotypes were HPV6（34.2%）,HPV11（26.5%）,HPV52（12.0%）,and HPV16（10.3%）.In group A,188（72.9%）were infected with a single HPV subtype,and 70（27.1%）with multiple HPV subtypes,and high-risk subtypes were detected 184 times（50.5%,184/364）.In group B,118（56.2%）were infected with a single HPV subtype,and 92（43.8%）with multiple HPV subtypes,and high-risk subtypes detected 249 times（63.8%,249/390）.The difference of frequency of HPV mixed subtype infection ratio and the percentage of highrisk subtypes between group A and B were statistically significant.Compared with group A,group B showed a decreased percentage of CD4＋,CD4＋/CD8＋,and an increased percentage of CD8＋.Compared with the normal control group,CD4＋,CD4＋/CD8＋of patients with CA in group A,especially group B showed statistically significant difference.Conclusion HPV high-risk subtypes of infection and high-risk subtypes with multiple infections,and the decreased cellular immune function are important factors in the recurrence of CA.

关 键 词：尖锐湿疣 人乳头瘤病毒亚型 细胞免疫功能 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]