金花茶提取物对MMTV-PyMT转基因小鼠自发乳腺癌的预防作用研究  

Chemical Constituents of Camelllia Nitidissima Tuyama Protects Against Tumor Formation in MMTV-PyMT Transgenic Mice

作　　者：侯晓莹[1] 杜鸿志[1] 伍瑶[1] 黄悦[1] 冯书云[1] 全兴萍 袁胜涛[1] 孙立[1] 

机构地区：[1]中国药科大学江苏省新药筛选重点实验室,江苏省药效评价与服务中心,江苏南京210009

出　　处：《现代生物医学进展》2017年第33期6417-6421,共5页Progress in Modern Biomedicine

基　　金：国家高技术研究发展计划(863计划,2014AA022208)

摘　　要：目的:考察金花茶二氯甲烷乙酸乙酯提取物对MMTV-Py MT转基因小鼠乳腺癌发生的实验预防作用。方法:MTT法检测金花茶二氯甲烷乙酸乙酯提取物对人乳腺癌细胞SKBR-3的体外增殖抑制作用;采用MMTV-Py MT转基因自发乳腺癌小鼠模型观察金花茶二氯甲烷乙酸乙酯提取物对小鼠体内肿瘤发生的预防作用。结果:金花茶二氯甲烷乙酸乙酯提取物对人乳腺癌细胞SKBR-3有显著地体外增殖抑制作用,其IC50为419.0±12.87μg/m L;转基因小鼠动物模型中,金花茶二氯甲烷乙酸乙酯提取物对小鼠肿瘤的发生和发展均有显著地抑制作用;模型组小鼠肿瘤平均发生数量为5.25个/只,给药组为3.75个/只;模型组组小鼠平均瘤重为0.47 g/只,给药组0.24 g/只。结论:金花茶二氯甲烷乙酸乙酯提取物对MMTV-Py MT转基因小鼠乳腺癌的发生有一定的预防作用。Objective： To investigate the effect of Chemical Constituents of Camelllia nitidissima Tuyama from methylene chlo- ride ethyl acetate（JHC）on protection against tumor formation in MMTV-PyMT transgenic mice. Methods： MTT assay was performed to evaluate the growth inhibitory rate of SKBR-3; The MMTV-PyMT transgenic mice model was performed to observe the protection effect in carcinogenesis of JHC in vivo. Results： JHC showed significantly inhibition effect on the proliferation of SKBR-3 and the IC50 419.03： 12.87 ~g/mL. In transgenic mice model, JHC shows significantly inhibitory effect in tumor formation and development; The average tu- mor number of model group is 5.25/mice, and the JHC treatment group is 3.75/mice; The average tumor weight of model group is 0.47g/mice, and the JHC treatment group is 0.24 g/mice. Conclusions： Chemical Constituents of Camelllia nitidissima Tuyamafrom Methylene chloride ethyl acetate can protect against the tumor formation in MMTV-PyMT transgenic mice.

关 键 词：金花茶 乳腺癌 MMTV-PyMT转基因小鼠 肿瘤预防 

分 类 号：R33[医药卫生—人体生理学] R737.9[医药卫生—基础医学]