TGF-β1在大鼠缺血再灌注肾损伤中的表达及地塞米松的影响  

The expression and significance of TGF-β1 and dexamethasone intervention in ischemia-reperfusion kidney injury

作　　者：张以勤[1] 林淑燕[1] 颜建华[1] 玄先法[1] 周丽娜[1] 王玉新[1] 

机构地区：[1]厦门市第二医院肾内科,福建厦门361021

出　　处：《国际泌尿系统杂志》2017年第6期900-904,共5页International Journal of Urology and Nephrology

基　　金：厦门市科技计划项目（3502220134030）;福建省卫生计生委中青年骨干人才培养项目（2014-ZAN-JC-44）

摘　　要：目的 通过建立缺血再灌注急性肾损伤（acute kidney injury,AKI）大鼠模型,观察转化生长因子β1（transforming growth factor β1,TGF-β1）在肾组织中的表达变化;观察地塞米松对缺血再灌注急性肾损伤的影响.方法 健康雄性SD大鼠随机分为假手术组（sham组,n=6）、缺血再灌注组（ischemia/reperfusion,I/R）（I/R组,n=6）、缺血再灌注＋地塞米松（dexamethasone,Dex）干预组（I/R＋ Dex组,n=6）.I/R组予右肾摘除,无损伤血管夹夹闭左肾动脉,45 min后恢复左肾供血制备AKI模型;sham组除未给予左肾动脉夹闭外,余操作同缺血再灌注组;I/R＋ Dex组同缺血再灌注组,在缺血前30 min予腹腔内注射地塞米松（4 mg/kg）,术后24 h处死动物,获取血清及肾组织标本.检测血清尿素氮、肌酐并观察肾组织病理学变化和评分,免疫组化及Western Blot检测TGF-β1含量的变化.结果 I/R组大鼠血清肌酐及尿素氮水平、肾组织TGF-β1表达比假手术组均显著升高,应用地塞米松干预后各项指标均显著改善.I/R组肾组织损伤最重,地塞米松干预组其次,假手术组基本正常.结论 缺血再灌注肾损伤后肾组织中TGF-β1表达升高,地塞米松可减少TGF-β1的表达,并且抑制炎症反应过程,减轻肾组织损伤.Objective To investigate the expression and significance of transforming growth factor-β1 （TGF-β1）and dexamethasone intervening in ischemia/reperfusion（I/R） rat kidney.Methods Sprague-Dawley（SD） rats were randomly assigned to the following groups,sham ＋ saline group （n =6）,I/R ＋ saline group （n =6） and I/R ＋ dexamethasone group （n =6）.Right kidney of rats in I/R group was removed,left renal artery was clamped until 45 min to restore blood supply.Only removed right renal and didn＆#39;t clamp the renal artery in sham group.Dexamethasone treatment group （I/R ＋ Dex） were intraperitoneally injected dexamethasone （4 mg/kg） before 30 min ischemia.All the animals were sacrificed to obtain blood samples and kidney tissue after 24 hours reperfusion.Serum urea nitrogen,creatinine and renal pathology were evaluated.TGF-β1 expression was determined by Western Blot and immunohistochemistry.Results Urea nitrogen and serum creatinine levels,TGF-β1 expression were significantly increased in I/R group as compared with sham group.However,they were significantly reduced after application of dexamethasone in I/R ＋ dexamethasone group.The damage of kidney and score of tubular injury was the highest in I/R group,followed in I/R ＋ dexamethasone group and the damage was the lightest in sham group.Conclusions TGF-β1 expression is up-regulated after ischemia reperfusion kidney injury.It is suggested the increase of TGF-β1 involved in renal injury.Dexamethasone can alleviate kidney injury by inhibiting the inflammatory and reducing the increase of TGF-β1.

关 键 词：再灌注损伤 肾疾病 大鼠 转化生长因子Β1 地塞米松 

分 类 号：R692[医药卫生—泌尿科学]