空斑试验在鼻病毒定量中的应用  

Application of plaque forming assay in the quantification of rhinovirus

作　　者：刘运可 陈爱欢[2] 孙丽红[2] 彭淑梅[1] 

机构地区：[1]广东省妇幼保健院儿科,广州510120 [2]广州医科大学附属第一医院广州呼吸疾病研究所儿科

出　　处：《国际病毒学杂志》2017年第6期380-383,共4页International Journal of Virology

摘　　要：目的 探讨空斑试验在鼻病毒定量过程中的应用.方法 Hela细胞单层在24孔板中融合达90％以上时,接种RV-1B,用琼脂作为半固体培养基孵育3-5 d后,甲醛固定、结晶紫染色,计算空斑.结果 利用Hela细胞进行空斑试验可以对RV-1B进行准确定量.结论 空斑试验为RV-1B定量提供一种相对有效方法,其中温度、PH、Mg2＋等可能影响实验结果的因素不可忽视.Objective To evaluate the application of plaque forming assay in the quantification of rhinovirus.Methods Rhinovirus was inoculated to Hela cell when the monolayer of cells achieved 90% fusion in 24-well plates.After culturing on semi-solid agar culture medium for 3-5 days,the cells were fixed with formaldehyde and were dyed with crystal violet.The number of plaque was counted.Results Rhinovirus could be quantified precisely by the assay with Hela cells.Conclusions Plaque assay could be a relatively effective method to determine the quantity of rhinovirus.The temperature,value ofpH and Mg2＋ may be the important factors during the laboratory test.

关 键 词：鼻病毒 空斑试验 HELA细胞 

分 类 号：R373[医药卫生—病原生物学]