四川省乐山市一起诺如病毒胃肠炎暴发的病原学特征分析  

The etiological characteristics of a gastroenteritis outbreak caused by norovirus in Leshan city of Sichuan province

作　　者：马小珍[1] 陈霞[2] 曹冉冉[1] 钟文辉 谢应国[2] 陈娜[1] 陈珍妍 任敏[1] 

机构地区：[1]四川省疾病预防控制中心,成都610041 [2]乐山市疾病预防控制中心 [3]五通桥区疾病预防控制中心 [4]四川大学华西公共卫生学院

出　　处：《国际病毒学杂志》2017年第6期401-404,共4页International Journal of Virology

摘　　要：目的 明确四川省乐山市一起学校急性胃肠炎暴发疫情的病原及基因型别,并对其基因特征进行初步研究.方法 采集暴发病例的粪便标本3份、呕吐物标本3份和健康儿童的粪便标本6份,采用实时荧光RT-PCR检测诺如病毒基因,诺如病毒阳性标本采用传统RT-PCR扩增并进一步测序分型.结果 诺如病毒实时荧光RT-PCR结果显示6份病例标本均检出诺如病毒阳性,基因序列比对和进化分析结果显示,6份均为G□.2基因型.结论 该起急性胃肠炎暴发疫情由G□.2诺如病毒引起.Objective To investigate the genotypes and genetic characteristics of the pathogens associated with an outbreak of acute gastroenteritis in Leshan city,Sichuan province.Methods Three stool samples and 3 vomitus samples from an acute gastroenteritis outbreak and 6 stool samples from healthy children were collected.Norovirus nucleic acid was detected by real-time reverse transcription polymerase chain reaction.The positive samples were amplified by conventional reverse transcription-polymerase chain reaction and the amplicons were sequenced.Results Six samples from the acute gastroenteritis outbreak were positive for norovirus.Sequence alignment and phylogenetic analysis showed that these 6 strains of virus belonged to G □.2 genotype.Conclusions This outbreak of acute gastroenteritis was caused by genotype G □.2 norovirus.

关 键 词：诺如病毒 胃肠炎 暴发 病原学特征 

分 类 号：R181.3[医药卫生—流行病学] R512.5[医药卫生—公共卫生与预防医学]