三氧化二砷对胶原诱导性关节炎模型大鼠的抗炎作用  

The anti-inflammatory action of arsenic trioxide in collagen induced arthritis model rats

作　　者：唐芳[1] 马武开[1] 陈玉婷 姚血明[1] 黄颖[1] 徐晖[1] 黄昆[1] 

机构地区：[1]贵阳中医学院第二附属医院风湿免疫科,贵州贵阳550001

出　　处：《中国老年学杂志》2017年第24期6005-6007,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81460727);贵州省社会公关课题(黔科合SY字(2013)3048号);国家中医药管理局重点建设专科项目(2012年);贵州省科技创新人才团队(黔科合人才团队(2013)4033);贵州省中医药管理局课题(QZYY2013-63)

摘　　要：目的探讨三氧化二砷(AS_2O_3)对牛Ⅱ型胶原诱导性关节炎(CIA)大鼠血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β水平的影响。方法将60只健康清洁级Wistar大鼠随机分为空白对照组、模型对照组、雷公藤对照组及AS_2O_3低、中、高剂量组,每组10只。除空白对照组外,其余各组建立CIA模型,观察AS_2O_3对CIA大鼠关节病理、关节炎指数的变化及对TNF-α、IL-1β表达的影响。结果 AS_2O_3能够抑制CIA大鼠关节肿胀,降低血清TNF-α、IL-1β水平,减轻关节滑膜细胞增生、滑膜层及滑膜下层淋巴细胞浸润,其中低、中剂量组疗效与雷公藤对照组相当(P>0.05),高剂量组优于雷公藤组(P<0.01)。结论 AS_2O_3能减轻CIA大鼠关节肿胀程度及关节滑膜淋巴细胞浸润,下调CIA大鼠血清致炎因子TNF-α、IL-1β的表达。Objective To observe the influence of arsenic trioxide（ AS2O3） on the level of serum tumor necrosis factor（ TNF）-α,interleukin（ IL）-1βin collagen induced arthritis（ CIA） model rats. Methods 60 healthy and clean Wistar rats were randomly divided into blank control,model control,tripterygium wilfordii（ Tw HF） control,AS2O3 high,medium and low dose treatment groups,each group with 10 rats. The changes of joint pathology and arthritis index in CIA rats were observed,and the levels of serum TNF-α,IL-1β were measured. Results AS2O3 inhibited the joint swelling,reduced the levels of serum TNF-α,IL-1β,and reduced the joint synovial cell hyperplasia and the lymphocyte infiltration in synovial layer and synovial lower layer. The curative effect in the AS2O3 low and medium dose treatment groups were comparable to those of Tw HF control group（ P〈0. 05）,the curative effect of high dose treatment group was better than that of Tw HF control group（ P〈0. 01）. Conclusions AS2O3 could alleviate the joint swelling degree and the joint synovial lymphocyte infiltration.

关 键 词：三氧化二砷 诱导性关节炎 肿瘤坏死因子-Α 白细胞介素-1Β 

分 类 号：R2-031[医药卫生—中西医结合]