异源表达CiRS基因通过生成白藜芦醇增强拟南芥的抗氧化能力  

Heterologous Expression of CiRS Gene Eenhances the Antioxidant Capacity of Arabidopsis by Increasing the Content of Resveratrol

作　　者：杨飞芸[1,2] 武燕燕 崔爽[1] 张秀娟 王瑞刚[1] 李国婧[1] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018 [2]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [3]内蒙古自治区生物技术研究院,呼和浩特010070

出　　处：《中国生物工程杂志》2017年第12期27-33,共7页China Biotechnology

基　　金：内蒙古自治区自然科学基金项目(2017MS0354;2015MS0385);内蒙古自治区科技创新团队计划(201503004)资助项目

摘　　要：白藜芦醇是一种具有多种医疗保健作用的植物芪类次生代谢产物,在农业、医药、食品和化妆品等领域受到广泛的关注。白藜芦醇合酶是白藜芦醇生物合成中唯一必需的关键酶,决定植物体内白藜芦醇的合成。将中间锦鸡儿中克隆到的CiRS基因(Gen Bank登录号MF678590)转入野生型拟南芥,实验结果显示:野生型的总黄酮含量明显高于转基因株系。HPLC测得转基因拟南芥中有白藜芦醇的生成,并且含量最高达335μg/g FW。紫外照射处理后转基因植物中丙二醛的积累量明显少于野生型。转基因植物提取物DPPH自由基清除能力均高于野生型。这些结果表明,中间锦鸡儿CiRS基因异源表达后利用与黄酮类物质的共同底物合成了白藜芦醇,使得转基因植物的抗氧化性增强。Resveratrol is a member of stilbenoids with disease resistant activity for plants and exhibits a wide range of important biological and pharmacological properties for human which has received extensive attention in the fields of agriculture, medicine, foods, cosmetics and so on. Resveratrol synthase （RS） is an exclusive necessary enzyme in the pathway of resveratrol biosynthesis which determines the synthesis of resveratrol in plants. CiRS, a RS gene isolated from Caragana intermedia, was transferred into Arabidopsis. Total flavonoids experimental results showed that the total flavonoids content of wild type was significantly higher than that of the transgenic lines. HPLC method was used to analyze the resveratrol content in transgenic plants which maximum content was 335 p^g/g FW. Accumulation of malondialdehyde （MDA） after UV treatment in transgenic plants was significantly less than the wild type. DPPH free radical scavenging ability of transgenic plants extraction was higher than the wild type. Taken together, these results indicated that the antioxidant activity of transgenic plants was enhanced with the expression of CiRS gene which synthesise resveratrol with flavonoid substrates.

关 键 词：白藜芦醇合酶 中间锦鸡儿 白藜芦醇 转基因拟南芥 

分 类 号：Q786[生物学—分子生物学]