松树-樟树混交林、纯林土壤微生物量碳、氮及多样性特征研究  

Soil microbial biomass C,N and diversity characteristics in pure and mixed forest of Pinus and Cinnamomun

作　　者：董敏慧[1] 张良成 文丽 吴子剑[4] 黄忠良[4] 张轩[4] 李辉[4] 覃晓莉[4] 

机构地区：[1]长沙环境保护职业技术学院,湖南长沙410000 [2]湖南常德国有林场,湖南常德415111 [3]湖南正信检测技术有限公司,湖南株洲412000 [4]湖南省林业科学院,湖南长沙410000

出　　处：《中南林业科技大学学报》2017年第11期146-153,共8页Journal of Central South University of Forestry & Technology

基　　金：湖南省科技厅重点项目(2015SK2041)

摘　　要：采用氯仿熏蒸浸提法比较了松树—樟树混交林与两种纯林(樟树林、松树林)土壤微生物量碳和氮的含量。应用Biolog技术,比较了混交林与纯林之间土壤微生物对不同碳源类型的利用情况。结果表明:松树—樟树混交林土壤微生物量碳和氮均高于两种纯林;混交林土壤微生物量碳含量占总有机碳含量的2.36%,土壤微生物生物量氮含量占全氮含量3.77%,均高于两种纯林;混交林土壤微生物平均颜色变化率(AWCD)高于两种纯林,且除碳水化合物之外,混交林对其他5类碳源的利用强度均高于两种纯林,这可能是导致混交林AWCD高于两种纯林的原因;混交林的Shannon多样性指数、Simpson优势度指数均高于两种纯林。土壤理化性质与土壤微生物生物量碳、氮及土壤微生物群落功能多样性的相关性分析,结果表明:3种森林与环境因子长期的共同作用导致土壤有机碳与土壤全氮含量的差异和土壤结构的差异分别是影响不同森林土壤微生物量碳、氮和微生物群落多样性差异的的主要原因。综上所述,松树—樟树混交林比松树纯林以及樟树纯林更有利于提高微生物的量及代谢活性。A chloroform fumigation extraction method was used to investigate in microbial biomass carbon and microbial biomass nitrogen in pure and mixed forest of Pinus and Cinnamomun. By using Biolog technology to compare the utilization of different carbon sources by rhizosphere microorganisms in pure and mixed forest of Pinus and Cinnamomun. The results showed that the concentration of microbial biomass carbon and microbial biomass nitrogen in mixed forest all higher than two pure forests; the ratios of microbial biomass carbon to total organic carbon and microbial biomass nitrogen to total nitrogen were highest in mixed forest, 2.36% and 3.77% respectively. The average well colour development （AWCD） of soil in mixed forest was higher than other two pure forests, and the ability of soil microbial organisms to metabolize carbon sources （except carbohydrates） were all higher than other two pure forests, which caused the AWCD in mixed forest higher than two pure forests. The Shannon index and Simpson index of mixed forest was both higher than that of other two pure forests. The correlation analysis of soil physical-chemical properties and microbial biomass and functional diversity showed that the differences in total organic carbon, total nitrogen concentrations and soil structure were affected by environment factors were the primary reason resulted in differences in soil microbial biomass and functional diversity in three forests. So mixed forest of Pinus and Cinnamomun has the most microbial biomass and activity.

关 键 词：土壤微生物多样性 微生物生物量碳 微生物生物量氮 功能多样性 

分 类 号：S714.3[农业科学—林学]