甘草酸对四氯化碳致小鼠肝纤维化及骨丢失的防治作用  

Effect of glycyrrhizin on CCl_4-induced liver cirrhosis and bone loss in mice

作　　者：邹丽宜[1] 吴铁[1] 崔燎[1] 

机构地区：[1]广东医学院药理学教研室,湛江524023

出　　处：《中国药理学通报》2002年第4期437-441,共5页Chinese Pharmacological Bulletin

基　　金：广东省卫生厅资助课题 ;NoA2 0 0 14 86

摘　　要：目的　研究甘草酸对肝纤维化小鼠骨丢失的防治作用。方法　用体积分数为 4 0 %的CCl4花生油皮sc 5wk致小鼠肝纤维化 ,观察与肝损伤相关的各种生化指标和肝脏病理切片结果以及测定小鼠右股骨的骨Ca2 + 量和其他骨微量元素以及骨羟脯胺酸的含量。结果　单用CCl4小鼠呈现典型的慢性肝损伤后肝纤维化的改变 ,骨重量和骨钙总量及骨羟脯胺酸的含量减少 (P <0 0 5 ) ,而甘草酸治疗组有明显的护肝及对抗骨丢失作用。AIM To study the relationship between hepatic fibrosis and osteoporosis and to determine the preventive effects of Glycyrrhizin on bone loss in mice. METHOD Forty PCR Mice were randomly divided into 4 groups. Group A mice were controls; Group B mice were given sc injection of 40% CCl 4 10 ml·kg -1 once per 5 days as fibrosis model group; Group C were given orally colchicine of 0 01 mg·kg -1 plus CCl 4 sc, Group D were given Glycyrrhizin(GL) of 100 mg·kg -1 orally plus CCl 4 sc. The four groups were treated for 42 days. The liver injury indexes were measured and the mineral elements and Hydroxyproline of the femur were determined. RESULT Compared with group A, the serum enzymes of alanine aminotransferase (ALT), aspartic acid aminotransferase (AST) were markedly increased and serum albumin (Alb) and A/G(Alb/(total protein Alb) were decreased significantly in group B whose liver slides also showed typical liver cirrhosis. The dried weight of femur in the cirrhosis mice was markedly reduced and the bone Calcium content and bone Hydroxyproline content were also significantly decreased in group B. Bone copper and bone magnesium were increased in group B. In group D, GL inhibited markedly the decrease of the serum enzymes and increased Calcium content and Hydroxyproline content of the bone compared with group B. The bone mass loss was prevented effectively by Glycyrrhizin. CONCLUSION The bone mass was lost in mice with chronic hepatic injury induced by CCl 4 and Glycyrrhizin can prevent bone loss which was accompanied by chronic hepatic injury in mice.

关 键 词：甘草酸 四氯化碳 小鼠 肝纤维化 骨丢失 防治 

分 类 号：R285.5[医药卫生—中药学] R575.2[医药卫生—中医学]