Arabidopsis CPR5 regulates ethylene signaling via molecular association with the ETR1 receptor  

作　　者：Feifei Wang Lijuan Wang Longfei Qiao Jiacai Chen Maria Belen Pappa Haixia Pei Tao Zhang Caren Chang Chun-Hai Dong 

机构地区：[1]College of Life Sciences, Qingdao Agricultural University [2]Department of Cell Biology and Molecular Genetics, University of Maryland

出　　处：《Journal of Integrative Plant Biology》2017年第11期810-824,共15页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31370322);the Shandong Development Program for Science and Technology (2015GNC110012) to CHD;the National Natural Science Foundation of China (31400247) to HP;a grant from the National Institutes of Health 1R01GM071855 to CC

摘　　要：The plant hormone ethylene plays various functions in plant growth, development and response to environmental stress. Ethylene is perceived by membranebound ethylene receptors, and among the homologous receptors in Arabidopsis, the ETR1 ethylene receptor plays a major role. The present study provides evidence demonstrating that Arabidopsis CPR5 functions as a novel ETR1 receptor-interacting protein in regulating ethylene response and signaling. Yeast split ubiquitin assays and bifluorescence complementation studies in plant cells indicated that CPR5 directly interacts with the ETR1 receptor. Genetic analyses indicated that mutant alleles of cpr5 can suppress ethylene insensitivity in both etr1-1 and etr1-2, but not in other dominant ethylene receptormutants. Overexpression of Arabidopsis CPR5 either in transgenic Arabidopsis plants, or ectopically in tobacco,significantly enhanced ethylene sensitivity. These findings indicate that CPR5 plays a critical role in regulating ethylene signaling. CPR5 is localized to endomembrane structures and the nucleus, and is involved in various regulatory pathways, including pathogenesis, leaf senescence, and spontaneous cell death. This study provides evidence for a novel regulatory function played by CPR5 in the ethylene receptor signaling pathway in Arabidopsis.The plant hormone ethylene plays various functions in plant growth, development and response to environmental stress. Ethylene is perceived by membranebound ethylene receptors, and among the homologous receptors in Arabidopsis, the ETR1 ethylene receptor plays a major role. The present study provides evidence demonstrating that Arabidopsis CPR5 functions as a novel ETR1 receptor-interacting protein in regulating ethylene response and signaling. Yeast split ubiquitin assays and bifluorescence complementation studies in plant cells indicated that CPR5 directly interacts with the ETR1 receptor. Genetic analyses indicated that mutant alleles of cpr5 can suppress ethylene insensitivity in both etr1-1 and etr1-2, but not in other dominant ethylene receptormutants. Overexpression of Arabidopsis CPR5 either in transgenic Arabidopsis plants, or ectopically in tobacco,significantly enhanced ethylene sensitivity. These findings indicate that CPR5 plays a critical role in regulating ethylene signaling. CPR5 is localized to endomembrane structures and the nucleus, and is involved in various regulatory pathways, including pathogenesis, leaf senescence, and spontaneous cell death. This study provides evidence for a novel regulatory function played by CPR5 in the ethylene receptor signaling pathway in Arabidopsis.

关 键 词：Arabidopsis CPR5 regulatesmolecular association withethylene signaling viathe ETR1 receptor 

分 类 号：Q943.2[生物学—植物学]