绿谷灵芝对人脐静脉内皮细胞ICAM-1和VCAM-1表达的影响  

The effects of ganoderma lucidum on expression of adhesion molecules ICAM-1 and VCAM-1 of HUVEC

作　　者：张红梅[1] 田辉凯[1] 姚小皓[1] 林志彬[1] 李学军[1] 

机构地区：[1]北京大学基础医学院药理学系,北京100083

出　　处：《中国药理学通报》2002年第4期460-464,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目 ;No 3 9770 2 86

摘　　要：目的　采用血清药理学的方法研究绿谷灵芝含药血清对氧化型低密度脂蛋白 (ox LDL)和糖化白蛋白诱导的内皮细胞表面血管细胞粘附分子 (VCAM 1)和细胞间粘附分子 (ICAM 1)表达的影响。方法　通过细胞ELISA和流式细胞仪技术检测细胞表面VCAM 1和ICAM 1表达的变化。结果　细胞ELISA结果显示 0 2 4 g·kg-1、0 72 g·kg-1绿谷灵芝连续灌胃给药 10d后取血分离得到的血清可降低糖化白蛋白诱导的内皮细胞表面VCAM 1和ICAM 1表达 (P <0 0 5 ) ,降低ox LDL诱导的ICAM 1表达 (P <0 0 5 ) ;流式细胞技术检测结果显示 0 72 g·kg-1绿谷灵芝含药血清可降低ox LDL和糖化白蛋白诱导的内皮细胞表面ICAM 1表达(P <0 0 5 ) ,0 12 g·kg-1绿谷灵芝含药血清和无药血清对内皮细胞表面粘附分子的表达无影响。结论　绿谷灵芝含药血清可降低糖化白蛋白和oxAIM To study the effects of sera derived from rats administrated Lugu Ganoderma Lucidum (LGL) on the expression of adhesion molecules ICAM 1 and VCAM 1 induced by oxidative low density lipoprotein (ox LDL) and glycated albumin METHODS The expression of ICAM 1 and VCAM 1 on the surface of endothelial cells was detected by endothelial cell enzyme linked immunosobent assay (ELISA) and flow cytometric technique RESULTS The results by cell ELISA demonstrated that the sera derived from the rats administrated 0 12 g·kg -1 p o of LGL did not show significant effects on theexpression of ICAM 1 induced by ox LDL, but the sera from the rats administrated 0 24 g·kg -1 , 0 72 g·kg -1 po for ten days produced significant effects of inhibiting the expression of ICAM 1 induced by ox LDL and the expression of ICAM 1 and VCAM 1 induced by glycated albumin The results by flow cytometric techniques showed that the sera from the rats administrated 0 72 g·kg -1 for ten days produced the same effect on ICAM 1 induced by ox LDL and glycated albumin CONCLUSION Lugu Ganoderma Lucidum can inhibit the expression of adhesion molecules on the surface of endothelial cells induced by ox LDL and glycated albumin.

关 键 词：药理学 实验研究 绿谷灵芝 人脐静脉 内皮细胞 ICAM-1 VCAM-1 

分 类 号：R285.5[医药卫生—中药学]