五味子多糖对原代大鼠肝细胞氧化应激损伤的保护机制研究  

Protective mechanism of schizandrae polysaccharide for oxidative stress in rat hepatocytes

作　　者：王荣[1] 刘莉[2] 张玲莉[3] 

机构地区：[1]武汉大学人民医院护理部,武汉430060 [2]武汉大学人民医院胸外科,武汉430060 [3]武汉大学人民医院药学部,武汉430060

出　　处：《实用药物与临床》2017年第12期1351-1354,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的探讨五味子多糖对原代大鼠肝细胞氧化应激的保护效应,并对其作用机制进行研究。方法体外培养原代大鼠肝细胞,分为空白对照组、H2O2诱导组、H2O2+低剂量五味子多糖组、H2O2+中剂量五味子多糖组、H2O2+高剂量五味子多糖组;CCK-8试剂检测大鼠肝细胞相对存活率,相关试剂盒检测NO、MDA含量,Western blot检测大鼠肝细胞中P67-phox、P47-phox、SOD1、HO-1、Rac1、p-Rac1蛋白表达水平。结果与H2O2诱导组相比,不同浓度五味子多糖可明显提升大鼠肝细胞的存活率(P<0.05);与H2O2诱导组相比,不同剂量五味子多糖可明显抑制细胞中NO、MDA的生成(P<0.05);Western blot检测显示,不同剂量五味子多糖可明显促进抗氧化蛋白HO-1、SOD1表达,抑制促氧化蛋白P67-phox、P47-phox的表达,并且五味子多糖显著抑制Rac1的磷酸化(P<0.05)。结论五味子多糖可明显改善大鼠肝细胞氧化应激损伤,其作用机制可能与抑制Rac1的磷酸化有关。Objective To investigate the protective effect of schizandrae polysaccharide for oxidative stress in rat hepatocytes,and study the molecular mechanism of schizandrae polysaccharide in inhibiting oxidative stress. Methods Primary rat hepatocytes were cultured in vitro,and the rat hepatocytes were divided into five groups： control group,H2O2-induced group,and low,medium and high-dose schizandrae polysaccharide groups. The effect of schizandrae polysaccharide on the relative survival rate of hepatocytes was observed using CCK-8 assay. The levels NO and MDA were measured by related kits. The expression levels of P67-phox,P47-phox,SOD1,HO-1,Rac1 and p-Rac1 in cells were determined by Western blot. Results Compared with H2O2-induced group,schizandrae polysaccharide could significantly improve the survival rate of hepatocytes（ P 〈 0. 05） and inhibit the levels of NO and MDA in hepatocytes（ P 〈 0. 05）; the expression levels of HO-1 and SOD-1 in all schizandrae polysaccharide groups were significantly increased（ P 〈 0. 05）,while the levels of P67-phox and P47-phox were significantly decreased（ P 〈 0. 05）. Moreover,the phosphorylation levels of Rac1 were significantly decreased in all schizandrae polysaccharide groups（ P 〈0. 05）. Conclusion Schizandrae polysaccharide can significantly inhibit the oxidative stress injury of hepatocytes and its underlying mechanism may involve suppressing the phosphorylation of Rac1.

关 键 词：五味子多糖 大鼠 肝细胞 氧化应激 

分 类 号：R285.5[医药卫生—中药学]