乌梅丸及其拆方对溃疡性结肠炎模型大鼠结肠黏膜病理变化的影响  

Effect of Wumei Pill and its Disassembled Formula on the Pathological Changes of Colonic Mucosa of Ulcerative Colitis Rats

作　　者：张静[1] 

机构地区：[1]郑州大学附属中心医院,河南郑州450007

出　　处：《中医学报》2017年第12期2404-2407,共4页Acta Chinese Medicine

基　　金：国家自然科学基金项目(81173273)

摘　　要：目的:探讨乌梅丸及其拆方对溃疡性结肠炎大鼠的治疗效果,分析其作用机制。方法:选用80只健康大鼠作为研究对象,其中10只作为正常组,其他70只使用2,4,6-三硝基苯磺酸/乙醇法制备溃疡性结肠炎模型。造模成功的70只大鼠,按照随机对照的原则分为7组,每组各10只。正常组与模型组给予2 m L生理盐水灌胃,西药组给予0.3 g·kg-1柳氮磺吡啶灌胃,乌梅丸组给予生药10.5 g·kg-1灌胃,乌梅丸辛开组给予生药3.3 g·kg-1灌胃,乌梅丸苦降组给予生药2.3 g·kg-1灌胃,乌梅丸辛开苦降组给予生药5.5 g·kg-1灌胃,乌梅丸补益组给予生药5.6 g·kg-1灌胃。对不同组大鼠结肠黏膜组织Jak、STAT3、白细胞介素-6(interleukin-6,IL-6)mRNA的表达进行检测,并对病理变化进行观察。结果:模型组Jak、STAT3、IL-6 mRNA相对表达量分别为(4.89±0.43)、(5.05±0.56)、(4.28±0.42),西药组(2.56±0.26)、(1.75±0.23)、(2.15±0.26),乌梅丸组(2.32±0.24)、(1.82±0.25)、(2.18±0.27),乌梅丸辛开苦降组(2.49±0.27)、(1.98±0.28)、(2.28±0.33)均明显低于模型组(P<0.05),与乌梅丸辛开组(4.59±0.31)、(4.86±0.49)、(3.92±0.44),乌梅丸苦降组(4.62±0.32)、(4.91±0.39)、(3.88±0.41),乌梅丸补益组(4.73±0.29)、(4.98±0.57)、(4.01±0.46)比较,差异均有统计学意义(P<0.05);正常组IL-6水平为(1.15±0.16)ng·g-1,明显低于模型组水平(3.62±0.23)ng·g-1(P<0.05);与模型组比较,各治疗组IL-6水平明显降低(P<0.05);其中西药组(1.78±0.18)ng·g-1、乌梅丸组(1.92±0.19)ng·g-1明显低于其他治疗组(P<0.05);模型组溃疡大小、炎症程度、病变深度分别为(2.59±0.52)mm2、(2.55±0.69)分、(2.50±0.65)mm,西药组分别为(1.52±0.36)mm2、(1.13±0.38)分、(1.15±0.36)mm,乌梅丸组分别为(1.55±0.34)mm2、(1.12±0.33)分、(1.13±0.32)mm,乌梅丸辛开组分别为(1.82±0.32)mm2、(1.48±0.49)分、(1.47±0.0.48)mm,乌梅丸苦降组分别为(1.89±0.36)mm2、(1.52±0.58)分、(1.51±0.52)mm,乌�Objective： To explore the curative effect of Wumei pill and its disassembled formula on ulcerative colitis rats and its mechanism analysis. Methods： 80 healthy rats were selected as the research objects. 10 of them were selected in the normal group,and the other 70 rats were treated with 2,4,6-three nitrobenzene sulfonic acid/ethanol method to prepare ulcerative colitis model.70 rats model were divided into 7 groups according to the randomized and controlled principle,with 10 rats in each group. Normal group and model group were given 2 mL saline,while the western medicine group were given 0. 3 g · kg-1 sulfasalazine orally.Wumei Pill group were given intragastric administration of 10. 5 g·kg-1 crude drug. Wumei Pill Xinkai group were given intragastric administration of 3. 3 g·kg-1 crude drug. Wumei Pill Kujiang group were given intragastric administration of 2. 3 g·kg-1 crude drug. Wumei Pill Xinkai Kujiang group were given intragastric administration of 5. 5 g·kg-1 crude drug. Wumei Pill Buyi group were given intragastric administration of 5. 6 g·kg-1 crude drug. The expressions of Jak,STAT3,IL-6 and mRNA in colonic mucosa tissues of rats in different groups were detected and pathological changes were observed. Results： Jak,STAT3,IL-6,mRNA expression levels in model group were（ 4. 89 ± 0. 43）,（ 5. 05 ± 0. 56）,（ 4. 28 ± 0. 42）; western medicine group（ 2. 56 ± 0. 26）,（ 1. 75 ± 0. 23）,（ 2. 15 ± 0. 26）; Wumei Pill group（ 2. 32 ± 0. 24）,（ 1. 82 ± 0. 25）,（ 2. 18 ± 0. 27）; Wumei Pill Xinkai Kujiang group（ 2. 49 ± 0. 27）,（ 1. 98 ± 0. 28）,（ 2. 28 ± 0. 33） was significantly lower than the model group（ P〈0. 05）. Compared with Wumei Pill Xinkai group（ 4. 59 ± 0. 31）,（ 4. 86 ± 0. 49）,（ 3. 92 ± 0. 44）,Wumei Pill Kujiang group（ 4. 62 ± 0. 32）,（ 4. 91 ±0. 39）,（ 3. 88 ± 0. 41）,and Wumei Pill Buyi group（ 4. 73 ± 0. 29）,（ 4. 98 ± 0. 57）,（ 4. 01 ± 0. 46）,there was statistically significant differences（ P〈

关 键 词：乌梅丸 拆方 溃疡性结肠炎 信号通路 结肠黏膜 大鼠 

分 类 号：R285.5[医药卫生—中药学]