柴朴汤对哮喘模型大鼠气道炎症及ERK/p38 MAPK信号通路的影响  

Effect of Chaipu Decoction on Airway Inflammation and ERK/p38 MAPK Signal Pathway in Rats with Asthma

作　　者：徐凤[1] 肖韩艳[1] 周淑芬[1] 毛艺纯 

机构地区：[1]牡丹江医学院第二附属医院

出　　处：《中国实验方剂学杂志》2018年第2期104-109,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省留学归国科学基金项目(LC2015H162)

摘　　要：目的：观察柴朴汤对哮喘模型大鼠气道炎症及细胞外信号调节激酶（ERK）／p38丝裂原活化蛋白激酶（p38MAPK）信号通路的影响。方法：sD大鼠随机分为空白组、模型组、柴朴汤低、中、高（0．75，1．5，3．0g·kg^-1）组，地塞米松组。采用卵蛋白（OVA）致敏激发建立大鼠哮喘模型。柴朴汤低、中、高剂量组于激发前0．5h给予相应剂量柴朴汤灌胃；地塞米松组于激发前0．5h给予地塞米松（0．005g·kg^-1）灌胃；模型组于激发前0．5h给予等体积生理盐水灌胃；空白组以生理盐水代替OVA进行腹腔注射及雾化吸入；隔日1次，共激发28d。观察各组大鼠支气管肺泡灌洗液（BALF）中细胞总数及分类细胞计数的变化；酶联免疫吸附法（ELISA）检测肺组织磷酸化ERK（p-ERK），磷酸化p38MAPK（p-p38MAPK）的活性；实时荧光定量PCR（Real-timePCR）分析检测ERK，p38MAPKmRNA的表达；蛋白免疫印迹法（Westernblot）检测ERK，p-ERK，p38MAPK，p-p38MAPK蛋白的表达；光镜下观察病理组织形态学变化及进行炎症评分。结果：模型组大鼠BALF中细胞总数和分类细胞计数；肺组织p-ERK，p-p38MAPK的活性，ERK，p38MAPKmRNA的表达，p-ERK，p-p38MAPK的表达，炎症评分均明显高于空白组（P〈0．01）；柴朴汤低、中、高剂量组和地塞米松组上述指标则明显低于模型组（P〈0．05，P〈0．01）。结论：柴朴汤可改善哮喘模型大鼠气道炎症，其机制可能与其抑制ERK／p38MAPK信号通路有关。Objective： To investigate the effect of Chaipu decoction on airway inflammation and extracellular signal-regulated kinase （ERK） /p38 mitogen-activated protein kinase （p38 MAPK） signal pathway in asthmatic rats. Method： A total of 60 SD rats were randomly divided into the blank control group, the model group, the low-dose Chaipu decoction group （0.75 g·kg-1）, the middle-dose Chaipu decoction group （1.5 g·kg-1）, the high-dose Chaipu decoction group （3.0 g·kg-1） and the dexamethasone group （0. 005 g·kg-1）. The rats were sensitized and challenged with ovalbumin （OVA） to establish the asthma model. The blank control group was given saline water; the model group was given saline water by garage at 0.5 h before being challenged; the low dose group, the middle dose group and the high dose group were given the corresponding doses of Chaipu decoction by garage at 0.5 h before being challenged; the Dexamethasone group was given dexamethasone by gavage at 0. 5 h before being challenged; the drugs were given once every other day, for 28 days. The changes in the total and differential cell counts in bronchoalveolar lavage fluid （BALF） of different groups were observed. The activity of phosphorylated ERK （p-ERK） and phosphorylated p38 MAPK （p-p38 MAPK） in lung tissues were detected by enzyme linked immunosorbent assay （ELISA）. ERK and p38 MAPK mRNA expressions in lung tissues were detected by Real-time PCR; expressions of ERK, p-ERK, p38 MAPK, and p-p38 MAPK in lung tissues were detected by Western blot; the changes in histopathology and inflammation scores were observed under optical microscope. Result： Compared with the blank control group, the changes in the total and differential cell counts in BALF, the activity of p-ERK and p-p38 MAPK, the expressions of ERK mRNA and p38 MAPK mRNA, the expressions of p-ERK and p-p38 MAPK in lung tissues were significantly increased in the model group （P 〈0.01 ）; all of the above indexes in the low-dose group, the middle-dose grou

关 键 词：柴朴汤 哮喘 炎症 细胞外信号调节激酶/p38丝裂原活化蛋白激酶(ERK/p38 MAPK)信号通路 

分 类 号：R285.5[医药卫生—中药学] R287[医药卫生—中医学]