乐胃饮加味方对胃癌前病变中NF-κB和STAT3共调控因子表达的影响  

Effects of modified Lewei Decoction on the co-regulated cytokines of NF-κB and STAT3 in precancerous lesions of gastric cancer

作　　者：朱飞叶 徐珊[2] 谢冠群[2] 

机构地区：[1]浙江中医药大学分析测试中心,杭州310053 [2]浙江中医药大学基础医学院,杭州310053

出　　处：《中华中医药杂志》2018年第1期350-352,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省中医药科技计划项目(No.2013ZQ009),浙江省自然科学基金项目(No.LQ17H270001),浙江中医药大学基础医学院“中医证的本质研究”创新团队(No.JCIT2016-3)

摘　　要：目的:研究乐胃饮加味方对胃癌前病变中NF-κB和STAT3共调控因子Bcl-x L、c-Myc、IL-6的影响。方法:将NF-κB(Rel A/p65)-PECFP和STAT3-PEYFP载体转染到SGC-7901人胃癌细胞内,给予LPS刺激,经乐胃饮加味方治疗后采用实时荧光定量PCR检测Bcl-x L、c-Myc、IL-6 m RNA表达,Western Blot检测Bcl-x L、c-Myc蛋白的表达。结果:LPS刺激后,Bcl-x L、c-Myc、IL-6 m RNA表达上调,Bcl-x L、c-Myc蛋白的表达亦上调,经乐胃饮加味方治疗后,Bcl-x L、c-Myc、IL-6 m RNA表达均明显下调,Bcl-x L、c-Myc蛋白的表达亦下降。结论:乐胃饮加味方能阻断炎性反应与恶性肿瘤正反馈回路中的相关因子,达到防治胃癌的作用。Objective： To observe the effects of modified Lewei Decoction on the co-regulated cytokines of Bcl-xL, c-Myc and 1L-6 of NF-κB and STAT3 in precancerous lesions of gastric cancer. Methods： The vectors of NF-B （RelA/p65）-PECFP and STAT3-PEYFP were transfected into SGC-7901 human gastric cancer cell. Modified Lewei Decoction was used after the cell was stimulated by LPS. Real time RT-PCR was applied to detect the mRNA expression of Bcl-xL, c-Myc and IL-6 in cell, Western Blot was used to detect the protein expression of Bcl-xL and e-Myc. Results： After LPS stimulation, the mRNA expression of Bcl- xL, c-Myc and IL-6 increased and the protein expression of Bcl-xL and c-Myc also increased, whereas the increased expressions were significantly weakened after modified Lewei Decoction intervention. Conclusion： Modified Lewei Decoction could treat precancerous lesions of gastric cancer and prevent gastric cancer by blocking the relevant factors in the inflammation-cancer positive feedback loop.

关 键 词：乐胃饮加味方 B细胞淋巴瘤-xL 原癌基因c—Mye 白细胞介素-6 炎性反应 癌前病变 

分 类 号：R285[医药卫生—中药学]