野百合碱诱导的肺动脉高压大鼠血清中瘦素和肺组织中瘦素受体的表达及意义  

Expression and significance of serum leptin and lung tissue leptin receptor in rats with pulmonary arterial hypertension induced by monocrotaline

作　　者：钱天赐 彭星星 梁先柱 阮富贵 孙江滨[1] 王海永[1] 

机构地区：[1]桂林医学院附属医院心血管外科,广西桂林541001 [2]桂林医学院第二附属医院心胸外科,广西桂林541001

出　　处：《重庆医学》2018年第1期11-13,共3页Chongqing medicine

基　　金：广西医药卫生自筹经费计划课题资助项目(Z2015397)

摘　　要：目的探讨瘦素及其受体(OB-R)在野百合碱(MCT)诱导的肺动脉高压(PAH)发生、发展中的意义。方法 15只SD大鼠被分为对照组(n=5)和两个实验组(n=10)。实验组经腹腔注射MCT建立大鼠PAH模型,对照组注射相同剂量的溶媒。两个实验组大鼠在注射MCT后分别于第2、4周抽取静脉血测量平均肺动脉压及右心肥厚指数,随后取出肺组织,观察肺血管的病理学变化,ELISA检测血清瘦素的表达,Western blot检测肺组织中OB-R的表达情况。结果与对照组相比,实验组大鼠的平均肺动脉压和右心肥厚指数明显升高(P<0.05);血清中瘦素及肺组织中OB-R的表达水平明显增加(P<0.05),且与平均肺动脉压呈正相关(r=0.912,P<0.05;r=0.861,P<0.05)。结论瘦素及OB-R可能在MCT诱导PAH的发生、发展中起重要作用。Objective To study the significance of leptin and its receptor（OB-R） in the occurrence and development of pul- monary arterial hypertension（PAH） induced by monocrotaline（MCT）. Methods Fifteen SD rats were divided into the control group（n=5） and two experimental groups（n= 10）. The experimental groups were intraperitoneally injected by MCT for construc-ting the PAH model and the control group was injected by the same dose of solvent groups. The venous blood was extracted at 2, 4 weeks after MCT injection in the two experimental groups. The mean pulmonary artery pressure（mPAP） and right ventricular hypertrophy index（RVHI） were measured and then -he lung tissue was removed. The pathological change of lung blood vessels was observed. The expression of serum leptin was detected by ELISA. The expression of OB-R in lung tissue was tested by Western blot. Results Compared with the control group,mPAP and RVHI in the experimental groups were significantly increased（P〈0. 05） ;the expression levels of serum leptin and lung tissue OB-R were increased significantly（P〈0. 05） .moreover, which were positively correlated with mPAP（r=0. 912 ,P〈0.05; r=0. 861, P〈0.05）. Conclusion Leptin and OB-R may play an important role in the occurrence and development of PAH induced by MCT.

关 键 词：瘦素 受体 瘦素 高血压 肺性 野百合碱 

分 类 号：R543.2[医药卫生—心血管疾病]