大鼠脊髓圆锥损伤后膀胱组织胰岛素受体的变化  

Experimental study of insulin receptor change from bladder after conus medullaris injury in rats

作　　者：于荣华[1] 侯春林[1] 赵建国[1] 林浩东[1] 宗海洋[1] 林耀发 

机构地区：[1]第二军医大学长征医院骨科,上海200003

出　　处：《中华显微外科杂志》2017年第6期560-563,共4页Chinese Journal of Microsurgery

基　　金：国家自然科学基金（81571207）

摘　　要：目的观察失神经支配大鼠膀胱组织胰岛素受体的变化规律，探讨胰岛素受体在膀胱失神经支配后退变过程中的作用。方法2016年1月至2017年6月．将40只SD大鼠随机分为实验组和对照组，每组20只。实验组大鼠破坏脊髓圆锥，对照组大鼠行假手术．两组分别于术后1d、1周、1个月、3个月处死，取膀胱组织．进行湿重测量和胰岛素受体免疫组织化学检测。结果术后1d、1周、1个月和3个月时的膀胱湿重．对照组大鼠分别为（0．089±0．022）g、（0．094±0．038）g、（0．106±0．112）g和（0．102±0．048）g，实验组大鼠分别为（0．092±0．026）g、（0．110±0．034）g、（0．538±0．098）g和（1．528±0．462）g，术后1d、1周时的膀胱湿重，实验组与对照组相比差异无统计学意义（P〉O．05）：术后1个月及3个月膀胱湿重．实验组大于对照组，差异有统计学意义（P〈0．05）。术后1d、1周、1个月、3个月膀胱组织中胰岛素受体阳性率．实验组分别为60％、100％、100％和80％（其中强阳性率为55％），对照组分别为20％、40％、40％和0％（无强阳性例数），实验组均远高于对照组，差异有统计学意义（P〈0．05）。结论膀胱组织失神经支配后胰岛素受体表达显著上升．恢复胰岛素受体功能可能是防止失神经支配后膀胱组织结构发生不可逆退变的有效方法。Objective To observe the change rules of insulin receptor from rat bladders after losing lower cental innervations so as to explore the function of insulin receptor in dencrvated bladder. Methods From January, 2016 to June, 2017, 40 Sprague-Dawley rats were randomly divided into experimental group and control group,each group was 20. The conus nledullaris of rats in experimental group were damaged, and rats in control group received sham operation. The rats in 2 groups were sacrificed at different time after surgery （l day, 1 week, 1 month, 3 mouths）. Bladder specimens were harvested to perform wet weight measurement and immunohistochemical detection of insulin receptor. Results Corresponding to 1 day, 1 week, l month and 3 months after surgery, the bladder wet weight of control group were （0.089±0.022）g, （0.094±0.038）g, （0.106±0.112）g and（0.102±0.048）g, and of experimental group were （0.092± 0.026）g, （0.110±0.034）g,（0.538±0.098）g and （1.528±0.462）g. One month and 3 months after surgery, bladder wet weight of experiment group were significantly increased as compared with those of control group （P〈0.05）. One day, 1 week, l month and 3 months after the operation, the positive rate of insulin receptor expression was 60%, 100%, 100% and 80%. And strongly positive rate was 55%. In control group, the positive rate of insulin receptor expression was 20%, 40%, 40%, and 0%. The expression of insulin receptor in experimental group was significantly higher than that in control group in every stage（P〈0.05）. Conclusion The expression of insulin receptor is significantly increased after denervation of bladder. The defections of insulin receptor might lead to apoptosis and muscle wasting after denervation. Restore insulin receptor function might be key point to prevent bladder tissue from irreversible damage.

关 键 词：脊髓圆锥 胰岛素受体 膀胱 失神经支配 

分 类 号：R651.2[医药卫生—外科学]