甘草干姜汤对变应性鼻炎大鼠IL-2、IL-5表达的影响  

Effect of Licorice Ginger Decoction on the Expression of IL-2 and IL-5 in Allergic Rhinitis Rats

作　　者：李秋席 任文凯[1] 陈远飞 曾祥光 张燕平[2] 吴遵秋 杨梅[1] 吴宁[1] 孙见飞 

机构地区：[1]贵州医科大学化学与生物化学实验室,贵州贵阳550025 [2]贵阳中医学院第二附属医院,贵州贵阳550003

出　　处：《贵州医科大学学报》2017年第12期1389-1392,共4页Journal of Guizhou Medical University

基　　金：贵州省科技厅科技计划课题[黔科合LH字(2015)7330;黔科合LG字(2012)009];贵州省高等学校大学生创新创业训练计划项目(201510660014;201710660017)

摘　　要：目的:观察甘草干姜汤对变应性鼻炎(AR)大鼠外周血清白细胞介素(IL-2)、白细胞介素-5(IL-5)表达的影响。方法:将48只大鼠随机分成4组,分别为正常组、模型组、西替利嗪组及甘草干姜汤组,后3组大鼠用卵清蛋白作为致敏原制作AR动物模型,造模成功后分别给予生理盐水、西替利嗪溶液、甘草干姜汤2~2.5 m L/只灌胃4周;观察各组大鼠灌药喂养期间的行为学变化,于末次灌胃结束时取血检测血清IL-2、IL-5水平,同时取鼻黏膜观察组织学变化。结果:西替利嗪组及甘草干姜汤组行为学变化接近正常组(P>0.05);与模型组比较,西替利嗪组和甘草干姜汤组IL-2明显上调、IL-5明显下调,差异有统计学意义(P<0.05),但同正常组比较,差异无统计学意义(P>0.05);大鼠鼻黏膜HE染色示,与模型组比较,西替利嗪组和甘草干姜汤组大鼠鼻黏膜组织结构逐渐修复,嗜酸性粒细胞明显减少。结论:甘草干姜汤可能通过调节IL-2及IL-5水平,从而改善AR大鼠的症状、减轻鼻黏膜炎症损伤。Objective： To observe the impact of licorice ginger decoction on peripheral blood interleukin-2（ IL-2） and interleukin-5（ IL-5） of allergic rhinitis rats. Methods： A total of 48 rats were randomly divided into saline control group,model group,cetirizine group and licorice ginger decoction group. All rats except the control group intraperitoneally injected with ovalbumin（ OVA） to establish allergic rhinitis model and the latter three groups were given 2 to 2. 5 m L/rat of physiological saline,cetirizine,licorice ginger decoction by gavage respectively for four weeks. Observing ethology change of all rats,and serum levels of IL-2 and IL-5 were detected at the last time of gavage; observing nasal mucosa tissues change. Results： Cetirizine group and licorice ginger decoction group ethology change was close to control group（ P〈0. 05）; comparing with model group,IL-2 level of cetirizine group and licorice ginger decoction group were obviously up-regulated while IL-5 obviously down-regulated,differences were statistically significant（ P〈0. 05）; comparing with control group,differences were not statistically significant（ P〈0. 05）; rat nasal mucosa HE staining indicated that nasal mucosa tissue structure were gradually repairing in cetirizine group and licorice ginger decoction group compared with model group,and eosinophilic granulocyte were obviously reduced. Conclusion： Licorice ginger decoction could improve AR rat symptoms and reduce the nasal mucosa inflammation injury by regulating levels of IL-2 and IL-5.

关 键 词：变应性鼻炎 甘草干姜汤 白细胞介素2 白细胞介素5 鼻黏膜 

分 类 号：R285.5[医药卫生—中药学]