两种口服促排卵药物对小鼠子宫内膜容受性影响的比较  

Effects of ovarian induction with Raloxifene versus Clomiphene Citrate on endometrial receptivity in mouse endometrium

作　　者：冼英杰 陈彩蓉[1] 梁小青 周秀琴[1] 伍世钢 颜秋霞[1] 赵晓英[1] 

机构地区：[1]广州医科大学附属第六医院,清远市人民医院生殖医学中心,广东省清远市511500

出　　处：《实用医学杂志》2017年第24期4053-4056,共4页The Journal of Practical Medicine

基　　金：广东省清远市科技计划项目(编号:2016B054)

摘　　要：目的比较口服促排卵药物雷洛昔芬(RAL)和克罗米芬(CC)促排卵对小鼠着床窗期子宫内膜容受性的影响。方法将48只昆明雌鼠随机分为对照组(NC)、CC组、RAL 180 mg组和RAL 240 mg组,灌胃给药后诱发排卵并合笼,妊娠后4.5 d取子宫内膜组织进行苏木素伊红(HE)染色实验及免疫组化实验。结果 (1)HE染色实验结果显示CC组小鼠子宫内膜发育欠佳,而其他3组的小鼠子宫内膜发育较成熟,组织形态好。(2)免疫组化实验结果显示,子宫内膜容受性标志物环氧合酶2(COX-2)和溶血磷脂酸受体3(LPAR3)在RAL 180 mg组、RAL 240 mg组与NC组的表达水平差异无显著性(P>0.05),而在CC组的表达水平显著弱于其他3组(P<0.05),使用RAL进行治疗不影响着床窗期小鼠子宫内膜容受性标志物COX-2和LPAR3的表达。结论 RAL具有与CC相似的促排卵作用,但对子宫内膜容受性的影响较CC小。Objective To investigate the effects of ovarian induction with raloxifene（RAL）versus clomi-phene citrate（CC）on the endometrial receptivity in mouse endometrium during perimplantation period. Methods 48 female Kun-ming mouse were randomly divided into four groups in equal number：RAL 240 mg group,RAL 180 mg group,CC group,natural conception group（NC）,all treated with ovulation induction after drug administration.Successfully mated female mouse were killed,and uterus samples were collected for HE stain-ing and immunohistochemistry. Results HE staining showed that the endometrial morphology in the RAL 180 mg group and RAL 240 mg group and NC group were better than that of CC group.The expressions of COX-2 and LP-AR3 in the RAL 180 mg group and RAL 240 mg group were similar to NC group,without significant difference among the three groups（P 〉 0.05）. But in the CC group,it was statistically significantly lower than other three groups（P〈0.05）,indicating ovarian induction with RAL did not decrease the expressions of COX-2 and LPAR3 in endometrium. Conclusion The mechanism of ovulation induction with RAL is similar to CC,but RAL has fewer adverse effects on the endometrial receptivity compared with CC.

关 键 词：雷洛昔芬 克罗米芬 环氧合酶2 溶血磷脂酸受体3 

分 类 号：R965[医药卫生—药理学]