Real-time Assessment of Cytosolic, Mitochondrial, and Nuclear Calcium Levels Change in Rat Pheochromocytoma Cells during Pulsed Microwave Exposure Using a Genetically Encoded Calcium Indicator  

作　　者：HU Shao Hua WANG Hui LU Li ZHOU Hong Mei WANG Chang Zhen GAO Ya Bing DONG Ji PENG Rui Yun 

机构地区：[1]Department of Experimental Pathology, Beijing Institute of Radiation Medicine, Beijing 100850, China [2]Department of Radiation Protection and Health Physics, Beijing Institute of Radiation Medicine, Beijing 100850, China [3]Department of General Surgery, Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai 200040, China

出　　处：《Biomedical and Environmental Sciences》2017年第12期927-931,共5页生物医学与环境科学（英文版）

基　　金：supported by the National Natural Science Foundation of China[No.31400716 and No.61401497]

摘　　要：Little information is available about the effects of exposure to pulsed microwaves on neuronal Ca^2＋ signaling under non-thermal conditions. In this study, rat pheochromocytoma （PC12） cells were exposed to pulsed microwaves for 6 min at a specific absorption rate （SAR） of 4 W/kg to assess possible real-time effects. During microwave exposure, free calcium dynamics in the cytosol, mitochondria, and nucleus of cells were monitored by time-lapse microfluorimetry using a genetically encoded calcium indicator （ratiometric-pericam, ratiometric-10ericam-mt,Little information is available about the effects of exposure to pulsed microwaves on neuronal Ca^2＋ signaling under non-thermal conditions. In this study, rat pheochromocytoma （PC12） cells were exposed to pulsed microwaves for 6 min at a specific absorption rate （SAR） of 4 W/kg to assess possible real-time effects. During microwave exposure, free calcium dynamics in the cytosol, mitochondria, and nucleus of cells were monitored by time-lapse microfluorimetry using a genetically encoded calcium indicator （ratiometric-pericam, ratiometric-10ericam-mt,

关 键 词：Real-time Assessment of Cytosolic MITOCHONDRIAL Nuclear Calcium Levels 

分 类 号：R736.6[医药卫生—肿瘤]