SD大鼠极端高温高湿环境暴露的实验研究  

The experimental research of the SD rats exposed to the high-temperature and high-humidity environment

作　　者：刘刚[1,2] 刘诗颖[2] 黄伍蓉[2] 简燚 呼永河[4] 吴丽娟[2] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031 [2]中国人民解放军成都军区总医院转化医学科/高湿医学全军重点实验室,成都610083 [3]中国人民解放军成都军区总医院病理科,成都610083 [4]中国人民解放军成都军区总医院中医科,成都610083

出　　处：《重庆医学》2017年第36期5052-5054,共3页Chongqing medicine

基　　金：四川省科技厅青年科技创新研究团队资助(2013TD0016)

摘　　要：目的调查极端高温高湿环境暴露对SD大鼠生存、血液生化指标的影响及对主要脏器的伤害。方法 26只SD大鼠分为常温常湿组和极端高温高湿组。观察SD大鼠在极端高温高湿环境中的死亡情况并于半数致死时间点捕杀动物进行血清生化指标测定和心、肺、肾、脑、肌肉和小肠6种组织的病理检测。结果极端高温高湿环境暴露可导致SD大鼠死亡,半数致死时间为48h;极端高温高湿组与常温常湿组相比,SD大鼠血清肌酐(Scr)显著性升高(P<0.05),血清碱性磷酸酶(ALP)、缺血修饰性清蛋白(IMA)明显下降(P<0.05),其他生化指标无显著性改变;极端高温高湿组SD大鼠心、肺、肾、小肠组织可见炎性细胞浸润,脑和肌肉组织未见明显病理性改变。结论极端高温高湿环境暴露对SD大鼠有明显伤害,可引起某些血液生化指标异常和心、肺、肾、小肠组织的炎性改变,甚至造成死亡。Objective To investigate the effect of extremely high temperature and high humidity exposure on the survival and blood biochemical indexes of SD rats and the to the major organs.Methods Twenty-six SD rats were divided into the normal temperature and humidity group and the extremely high-temperature and high-humidity group.The mortality of SD rats in extremely high-temperature and high humidity environment was observed,and at the median lethal time,the living rats were undergoing the tests of serum biochemical indicators and the pathological examination of the heart,lung,kidney,brain,muscles and intestinal tissues.Results The exposure to the extremely high-temperature and high-humidity environment could lead to the death of SD rats,the median lethal time was 48 h.The serum creatinine（Scr）increased significantly（P〈0.05）,and the serum alkaline phosphatase（ALP）and ischemia modified albumin（IMA）decreased significantly（P〈0.05）when compared with the normal temperature and humidity group,and there was no significant difference in other biochemical indicators.The inflammatory cell infiltration was observed in heart,lung,kidney and intestines,and there were no obvious pathological changes in brain and muscle.Conclusion The exposure to the extremely high-temperature and high-humidity environment has obvious lesion on SD rats,it can lead to the abnormality of some biochemical indicators,the inflammatory changes in heart,lung,kidney and intestines,and even death.

关 键 词：高温 高湿 环境暴露 大鼠 Sprague-Dawley 生物学标记 病理学 临床 

分 类 号：R594[医药卫生—内科学]