盐酸法舒地尔脂质体的制备  

The preparation of fasudil hydrochloride liposomes

作　　者：胡艳秋[1] 胡俊华[2] 孙长海[1] 张云杰[1] 柴佳丽[1] 刘文文 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]佳木斯市疾病预防控制中心,黑龙江佳木斯154007

出　　处：《黑龙江医药科学》2017年第6期28-30,共3页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省大学生创新创业训练计划项目;编号:201510222006;佳木斯大学基础研究类(自然类)面上项目;编号:JMSUJCMS2016-068;黑龙江省卫生计生委科研课题;编号:2016-323

摘　　要：目的:制备盐酸法舒地尔脂质体,探讨制备盐酸法舒地尔脂质体的最优处方及最佳制备工艺。方法:采用醋酸钙梯度法制备盐酸法舒地尔脂质体。以包封率为考察指标,在单因素实验基础上,通过正交试验优化最优处方及最佳制备工艺,采用紫外分光光度法对盐酸法舒地尔脂质体包封率进行测定。结果:最终确定制备盐酸法舒地尔脂质体的最优处方及最佳制备工艺为药脂比1:20、孵育时间1.5h、醋酸钙浓度100mmol/L、孵育温度45℃,所制备的盐酸法舒地尔脂质体包封率达80.94%。结论:醋酸钙梯度法制备盐酸法舒地尔脂质体方法可行,所制得载药脂质体包封率达到制剂学要求,且重现性好。Objective: To prepare and discuss optimal prescription and the best preparation process for fasudil hydrochloride liposomes. Method: The fasudil hydrochloride lipsomes were prepared by calcium acetate gradient method. Based on the single factor experiment,the optimum formulation and optimum preparation were optimized by orthogonal design. The encapsulation efficiency of fasudil hydrochloride lipsomes were determined by ultraviolet-visible spectrophotometry. Results: The optimum formulation and optimum preparation for fasudil hydrochloride liposomes were as follows: the proportion of fasudil hydrochloride and lipids in weight was 1: 20,incubation time was 1. 5 h,the concentration of calcium acetate was 100 mmol/L,incubation temperature was 45°C. The encapsulation efficiency of fasudil hydrochloride liposomes were 80. 94%. Conclusions: Calcium gradient preparation of fasudil hydrochloride liposome method is feasible,the prepared drug loaded liposomes reached the requirements of pharmaceutics,and had good reproducibility.

关 键 词：盐酸法舒地尔 脂质体 醋酸钙梯度法 包封率 

分 类 号：R285.5[医药卫生—中药学]