原发性干燥综合征相关间质性肺疾病患者血清活性氧、转录因子κB及转化生长因子β_1水平变化及其临床意义研究  

Changes and Clinical Significance of Serum Levels ROS, NF-κB and TGF-β1in Patients with Primary Sjogren' s Syndrome Associated Interstitial Lung Disease

作　　者：白淑荣[1] 沈乐[1] 陈乾 杨静[1] 耑冰[1] 王艳[1] 李萍[1] 杨朝[1] 郭东更[2] 杨青兰[1] 

机构地区：[1]宁夏回族自治区人民医院呼吸内科,宁夏银川市750001 [2]宁夏回族自治区人民医院风湿科,宁夏银川市750001

出　　处：《实用心脑肺血管病杂志》2017年第11期42-45,共4页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

基　　金：宁夏自然科学基金资助项目(NZ15184)

摘　　要：目的观察原发性干燥综合征相关间质性肺疾病(pSS-ILD)患者血清活性氧(ROS)、转录因子κB(NF-κB)及转化生长因子β_1(TGF-β_1)水平变化,并探讨其临床意义。方法选取2015年7月—2017年6月宁夏回族自治区人民医院呼吸科和风湿科收治的女性原发性干燥综合征(pSS)患者94例,其中单纯pSS患者46例作为pSS组,pSS-ILD患者48例作为pSS-ILD组;另选取同期体检健康女性50例作为对照组。采用免疫印迹法检测抗干燥综合征A(SSA)抗体、抗干燥综合征B(SSB)抗体、抗Robert52(Ro52)抗体,采用酶联免疫吸附试验(ELISA)检测血清ROS、NF-κB及TGF-β_1水平;血清NF-κB水平与pSS-ILD患者血清ROS及TGF-β_1水平的相关性分析采用Pearson相关性分析。结果 pSS组和pSS-ILD组患者抗SSA抗体、抗SSB抗体及抗Ro52抗体阳性率高于对照组(P<0.05);pSS组和pSS-ILD组患者抗SSA抗体、抗SSB抗体及抗Ro52抗体阳性率比较,差异无统计学意义(P>0.05)。pSS组和pSS-ILD组患者血清ROS、NF-κB及TGF-β_1水平高于对照组,pSS-ILD组患者血清ROS、NF-κB及TGF-β_1水平高于pSS组(P<0.05)。Pearson相关性分析结果显示,血清NF-κB水平与pSS-ILD患者血清ROS(r=0.95)及TGF-β_1(r=0.98)水平呈正相关(P<0.05)。结论 pSS-ILD患者血清ROS、NF-κB及TGF-β_1水平明显升高,且血清NF-κB水平与血清ROS、TGF-β_1水平有关,推测ROS-NF-κB-TGF-β_1信号通路可能与pSS-ILD的发生有关。Objective To observe the changes of serum levels of ROS,NF-κB and TGF-β1 in patients with primary Sjogren＇s syndrome associated interstitial lung disease（ pSS-ILD）,to explore the clinical significance. Methods From July2015 to June 2017,a total of 94 female patients with primary Sjogren＇ s syndrome（ pSS） were selected in the Departments of Respiratory Medicine and Rheumatology,the People＇ s Hospital of Ningxia Hui Autonomous Region,thereinto 46 patients with isolated pSS were served as A group,the other 48 patients with pSS-ILD were served as B group; meanwhile a total of 50 healthy women were selected as control group. Western blotting method was used to detect anti-SSA anti-body,anti-SSB antibody and anti-Ro52 antibody,enzyme-linked immunosorbent assay was used to detect the serum levels of ROS,NF-κB and TGF-β1;Pearson correlation analysis was used to analyze the correlations of serum NF-κB level with serum levels of ROS and TGF-β1 in patients with pSS-ILD. Results Positive rates of anti-SSA antibody,anti-SSB antibody and anti-Ro52 antibody in A group and B group were statistically significantly higher than those in control group（ P〈0. 05）, while no statistically significant differences of positive rate of anti-SSA antibody,anti-SSB antibody or anti-Ro52 antibody was found between A group and B group（ P〉0. 05）. Serum levels of ROS,NF-κB and TGF-β1 in A group and B group were statistically significantly higher than those in control group,meanwhile serum levels of ROS,NF-κB and TGF-β1 in B group were statistically significantly higher than those in A group（ P〈0. 05）. Pearson correlation analysis results showed that,serum NF-κB level was positively correlated with serum level of ROS（ r = 0. 95）,of TGF-β1（ r = 0. 98） in patients with pSS-ILD,respectively（ P〈0. 05）. Conclusion Serum levels of ROS, NF-κB and TGF-β1 are significantly elevated in patients with pSS-ILD, and serum NF-κB level is correlated with serum level of ROS,of TGF-β1,respectively,it is p

关 键 词：干燥综合征 肺疾病 间质性 活性氧 转录因子κB 转化生长因子Β1 

分 类 号：R593.22[医药卫生—内科学]