脂肪酸结合蛋白4基因启动子rs77878271多态性与颈动脉粥样硬化的关系研究  

Relationship between FABP4 Gene Promoter rs77878271 Polymorphism and Carotid Atherosclerosis

作　　者：焦荣红[1] 于明月[1] 张明明[2] 牛慧敏[1] 刘欣[1] 

机构地区：[1]河北省人民医院超声科,河北省石家庄市050051 [2]河北省人民医院检验科,河北省石家庄市050051

出　　处：《实用心脑肺血管病杂志》2017年第11期46-50,共5页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

基　　金：河北省重点课题计划项目(20150137)

摘　　要：目的探究脂肪酸结合蛋白4(FABP4)基因启动子rs77878271多态性与颈动脉粥样硬化的关系。方法选取2013年10月—2014年9月在河北省人民医院就诊的颈动脉粥样硬化患者580例,采用TaqMan SNP基因分型技术检测rs77878271多态性;采用实时荧光定量聚合酶链反应(qPCR)检测FABP4 mRNA相对表达量,采用酶联免疫吸附试验(ELISA)检测FABP4蛋白。rs77878271基因型与颈动脉中层内膜厚度(IMT)的关系分析采用多元线性回归分析,与颈动脉斑块的关系分析采用多因素Logistic回归分析。结果本组患者中携带TT基因型者占88.6%(514/580)、携带TC基因型者占10.0%(58/514)、携带CC基因型者占1.4%(8/58)。不同rs77878271基因型患者随访期间心血管事件发生率比较,差异无统计学意义(P>0.05)。不同rs77878271基因型患者男性比例、年龄、吸烟率、肥胖发生率、高血压发生率、糖尿病发生率、使用降压药物者所占比例、使用降糖药物者所占比例、使用降脂药物者所占比例、使用抗栓药物者所占比例及总胆固醇(TC)、高密度脂蛋白(HDL)、三酰甘油(TG)、空腹血糖(FBG)、空腹胰岛素(INS)、C反应蛋白(CRP)水平比较,差异均无统计学意义(P>0.05);不同rs77878271基因型患者体质指数(BMI)、IMT、颈动脉斑块者所占比例及低密度脂蛋白(LDL)水平比较,差异均有统计学意义(P<0.05)。多元线性回归分析结果显示,rs77878271基因型与颈动脉粥样硬化患者颈动脉IMT有关(β=0.23,P<0.05);多因素Logistic回归分析结果显示,rs77878271基因型是颈动脉粥样硬化患者颈动脉斑块的影响因素[OR=3.02,95%CI(1.46,7.97),P<0.05]。TC基因型患者FABP4 mRNA相对表达量和蛋白相对表达量均低于CC基因型患者(P<0.05)。结论 FABP4基因启动子rs77878271多态性与颈动脉粥样硬化程度有关。Objective To investigate the relationship between FABP4 gene promoter rs77878271 polymorphism and carotid atherosclerosis. Methods A total of 580 patients with carotid atherosclerosis were selected in the People＇ s Hospital of Hebei Province,Taq Man SNP genotyping technique was used to detect the FABP4 gene promoter rs77878271 polymorphism; real-time fluorescence quantitative PCR was used to detected the relative expression quantity of FABP4 mRNA,enzyme-linked immunosorbent assay was used to detect the relative expression quantity of FABP4 protein. Multivariate linear regression analysis was used to analyze the influencing factors of carotid IMT,meanwhile multivariate Logistic regression analysis was used to analyze the influencing factors of carotid plaques. Results Of the 580 patients,514 cases were TT genetype（ accounting for 88. 6%）,58 cases were TC genetype（ accounting for 10. 0%）,8 cases were CC genetype（ accounting for 1. 4%）. No statistically significant differences of incidence of diovascular events was found in patients with different genetypes of FABP4 gene promoter rs77878271 during the follow-up（ P〉0. 05）. No statistically significant differences of male proportion,age,smoking rate,incidence of fat, hypertension or diabetes, proportion of patients using antihypertensive drugs, antidiabetic drugs, lipid-lowering drugs or antithrombotic drugs,TC,HDL,TG,FBG,INS or CRP was found in patients with different genetypes of FABP4 gene promoter rs77878271（ P〉0. 05）, while there were statistically significant differences of BMI, carotid IMT,proportion of patients with carotid plaques and LDL in patients with different genetypes of FABP4 gene promoter rs77878271（ P〈0. 05）. Multivariate linear regression analysis results showed that, genetypes of FABP4 gene promoter rs77878271 was significantly correlated with carotid IMT in patients with carotid atherosclerosis（ β = 0. 23,P〈0. 05）; multivariate Logistic regression analysis results showed that,genetypes of FABP4 gene promoter

关 键 词：颈动脉硬化 脂肪酸结合蛋白4 rs77878271 多态性 单核苷酸 

分 类 号：R543.4[医药卫生—心血管疾病]