日本红枫外植体的选择及启动培养初步研究  

Preliminary Study on the Selection and Initial Culture of Acer palmatum 'Atropurpureum' Explants

作　　者：舒婷[1] 高小坤[1] 罗文熠 陈齐明 赖文胜 张文元 

机构地区：[1]福建省林业科技试验中心,福建南靖363600 [2]福建省鑫闽种业有限公司,福建福清350301 [3]福建省林木种苗总站,福州350003

出　　处：《安徽林业科技》2017年第6期26-28,32,共4页Anhui Forestry Science and Technology

基　　金：福建省林业厅花卉攻关项目<日本彩叶枫品种引进创新与产业化关键技术研发>;福建省林业厅科技项目<新优观赏树木引种及高效繁育技术研究>

摘　　要：本文对日本红枫不同部位外植体、不同消毒处理、褐化处理以及启动培养基进行研究。结果表明,以日本红枫茎尖为外植体,70%酒精15 s和0.1 g/L升汞6 min进行灭菌效果最好,污染率最低,萌发率最高,可达92%。在培养过程中,适当添加抗坏血酸能有效抑制褐化的形成。最佳启动培养基为MS+6BA 1.0 mg/L+NAA 0.1 mg/L+蔗糖30g/L,抽芽率可达到84.9%。In this paper, a study was made on explants of different parts of Acer palmatum ＇atropurpureum% their different disinfection treatments, treatments against browning and initial culture media. The results showed that using the stem tips as explants, treated with 70% alcohol for 15s and 0.1g·L^-1 mercury bichloride for 6min for sterilization could achieve the best effects with the lowest contamination rate and the highest germination rate of up to 92%. In the course of culture, proper addition of ascorbic acid could effectively inhibit browning. The best initial medium was MS＋6BA1.0mg·L^-1＋NAA0.1mg·L^-1＋, sucrose, 30g·L^-1. The germination rate could reach 84.9%.

关 键 词：日本红枫 外植体 褐化 启动培养基 

分 类 号：S722.37[农业科学—林木遗传育种]