健脾复方对结直肠癌细胞增殖及侵袭迁移的影响  

Effect of Jian Pi compound on proliferation,invasion,and migration of colorectal cancer cells

作　　者：臧明泉 任建琳[1] 靖琳 陶灵佳 桂奕文 陈文婷 李宁[1] 

机构地区：[1]上海中医药大学附属市中医医院肿瘤科,200071 [2]上海市漕河泾街道社区卫生服务中心中医科,200235

出　　处：《国际消化病杂志》2017年第6期382-385,391,共5页International Journal of Digestive Diseases

基　　金：国家自然科学基金(81473628);上海市自然科学基金(14ZR1438900)

摘　　要：目的探讨健脾复方对结直肠癌细胞增殖、侵袭和迁移及MALAT1表达的影响。方法通过CCK-8法检测健脾复方对LoVo细胞增殖的影响;根据CCK-8法检测结果,设立空白对照组和健脾复方高、中、低浓度组,然后采用Transwell实验检测健脾复方对LoVo细胞侵袭和迁移能力的影响;采用RT-PCR检测健脾复方对LoVo细胞长链非编码RNA MALAT1表达的影响。结果 LoVo细胞的生长抑制率与健脾复方浓度呈正相关;侵袭实验显示与对照组相比,到达侵袭小室底部的LoVo细胞数量随着健脾复方浓度增高而减少;Transwell迁移实验显示健脾复方能够抑制LoVo细胞的迁移能力,并且随着健脾复方浓度的增高,其迁移能力显著降低。健脾复方亦能对MALAT1表达起抑制作用。结论健脾复方可抑制结直肠癌细胞增殖、侵袭和转移并降低MALAT1表达,其抑制LoVo细胞侵袭转移的机制可能与降低MALAT1表达有关。Objective This paper intents to investigate the influence of Jian Pi compound on the proliferation,invasion,migration,and the MALAT1 expression of colorectal cancer cells.MethodsThe CCK-8 was chosen to count the proliferation state of LoVo cells after treated with Jian Pi compound.The control group,the high,the medium,and the low concentration groups of Jian Pi compound were established according to the results obtained by CCK-8.Then,the migration and invasion of LoVo cells were measured by the Transwell.Besides the expression of long noncoding,MALAT1 in the LoVo cells was tested by RT-PCR.Results The growth inhibition rate of LoVo cells was positively correlated with the concentration of Jian Pi compound.Compared with the control group,the number of LoVo cells which reached the bottom of Transwells decreased significantly with the increase of the concentration of Jian Pi compound in Transwell invasion testes.Besides,the Transwel migration test also indicated that the migration ability of LoVo was inhibited by Jian Pi compound,and the migration ability of LoVo notablely declined as the concentration rises.In addition the expression of MALTA1 was also restrained by Jian Pi compound.Conclusions Jian Pi compound could inhibit the proliferation,invasion,migration of colorectal cancer cells.The inhibition mechanism may be connected with the depression of MALAT1.

关 键 词：结直肠癌 侵袭 转移 迁移 MALAT1 增殖 

分 类 号：R285[医药卫生—中药学]