基于代谢组学的芩百清肺浓缩丸治疗支原体肺炎小鼠的作用机制研究  

Study on mechanism of Qinbai Qingfei Concentrated Pellets in treatment of mycoplasma pneumonia mice model based on metabolomics

作　　者：魏文峰 刘烨 霍金海 王伟明 

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150036

出　　处：《中草药》2017年第24期5211-5216,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81374045);黑龙江省应用技术研究与开发计划(PB15F005);哈尔滨市青年科技创新人才基金项目(2016RAQYJ198)

摘　　要：目的研究芩百清肺浓缩丸治疗支原体肺炎的作用机制。方法采用超高效液相色谱与串联四级杆飞行时间质谱(UPLC-Q-TOF-MS)联用技术的代谢组学方法,分析肺炎支原体感染小鼠肺组织中内源性化合物的变化。采用Progenisis QI软件进行色谱峰识别及匹配,并采用主成分分析(PCA)和偏最小二乘-判别分析(PLS-DA)对获得数据进行降维,通过分析对不同组间分离贡献度较大(VIP>1,P<0.05)化合物的串联质谱数据,经HMDB等数据库检索,确定潜在生物标志物。结果从肺组织中共鉴定出视黄醛、全反式维甲酸、焦谷氨酸、白三烯C4、维生素A、花生四烯酸、前列腺素I2等20个潜在生物标志物。与模型组比较,芩百清肺浓缩丸对20个潜在生物标志物均具有回调作用。结论芩百清肺浓缩丸通过影响视黄醇代谢、亚油酸代谢、花生四烯酸代谢等通路发挥治疗支原体肺炎的作用。Objective To study the mechanism of Qinbai Qingfei Concentrated Pellets（QQCP） in the treatment of mycoplasma pneumonia pneumonia. Methods UPLC-Q-TOF-MS was applied to detect the change of endogenous substances in the lung tissue of mycoplasma pneumonia mice model after taking decoction of QQCP orally. Progenisis QI was adopted to identify and match the peak, and principal compoment analysis（PCA） and partial least squares discriminant analysis（PLS-DA） were used to reduce data dimension. Through the analysis of tandem mass spectrum data of compound that had larger contribution to the separation among different groups（VIP 1, P 0.05） and the HMDB database retrieval to identify potential biomarkers. Results Twenty potential biomarkers were identified from lung tissue as retinal, all-trans-retinoic acid, pyroglutamic acid, leukotriene C4, vitamin A, arachidonic acid, and prostaglandin I2. Compared with model group, QQCP group had callback function of 20 potential biomarkers. Conclusion QQCP play a role of treatment for mycoplasma pneumonia by affecting retinol metabolism, linoleic acid, and arachidonic acid metabolism. These results indicated that QQCP had the characteristics of multi-pathway, multi-target and overall regulation in the treatment of mycoplasmal pneumoniae pneumonia.

关 键 词：芩百清肺浓缩丸 肺炎支原体 代谢组学 主成分分析 偏最小二乘-判别分析 

分 类 号：R285.5[医药卫生—中药学]