荧光实时定量PCR研究GA负调控因子基因在棉花纤维不同发育时期的表达模式  

Analysis of the Expression Pattern of GA Insensitive Regulator Gene in Cotton Fiber Development by Realtime PCR

作　　者：易小平[1] 谢翔[1] 廖文彬[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带农业科学》2017年第10期36-40,共5页Chinese Journal of Tropical Agriculture

基　　金：2014年公益性行业科研专项(No.201403075):南繁区生物安全监测预警及控制关键技术研究与示范

摘　　要：棉花纤维发育经历起始期、延伸期、次级细胞壁加厚期、脱水期共4个发育时期。为了了解GA负调控因子基因在整个纤维发育时期的调节作用,采用敏感的实时定量PCR对GA负调控因子基因Gh RGL在棉花纤维不同发育时期中的转录水平进行了定量研究,通过提取来源于不同纤维发育阶段[包括-3~0 DPA(开花后)胚珠,3 DPA胚珠,5 DPA胚珠,10 DPA胚珠,15 DPA纤维,25 DPA纤维]的样品总RNA,探讨不同发育时期Gh RGL基因的表达模式。结果显示,Gh RGL基因在所有被选的样品中都有表达,其中在5 DPA胚珠和10 DPA胚珠中转录水平较高,在10 DPA胚珠中表达水平最高,在-3~0 DPA胚珠、3 DPA胚珠、15 DPA纤维、25 DPA纤维中的表达水平较低,表明Gh RGL基因在纤维伸长期表达水平较高,暗示Gh RGL可能对纤维伸长起着重要作用。Cotton fibers, which are highly elongated epidermal cells that grow from the seed integument, undergo a developmental process that includes cell fate determination, initiation, elongation, specialization, and finally, programmed cell death. Transcript levels of GhRGL at different stages of cotton fiber development were quantified and analyzed by sensitive and real-time PCR technique to understand the expression pattern of GhRGL gene at all stages of cotton fiber development. Total RNA samples were extracted from different stages of cotton ovules and fibers including -3-0 DPA ovules, 3 DPA ovules, 5 DPA ovules, 10 DPA ovules, 15 DPA fibers, and 25 DPA fibers to analyze the expression pattem of GhRGL gene in cotton fiber development. The results showed that the GhRGL gene was expressed in all selected cotton samples. Higher levels of GhRGL mRNA were detected in 5 DPA and 10 DPA ovules, and the highest levels of GhRGL mRNA were detected in 10 DPA ovules. Lower levels of mRNA were detected in -3-0 DPA ovules, 3 DPA ovules and 15 DPA fibers, and 25 DPA fibers, gene was expressed at the highest levels at the fiber elongation stage, suggesting the important role of GhRGL in cotton fiber elongation.

关 键 词：荧光实时定量PCR 棉花纤维发育 GA负调控因子基因 表达模式 

分 类 号：Q943.2[生物学—植物学]