深圳地区甲氧西林耐药金黄色葡萄球菌耐药性及分子流行病学特征  

Antibiotic resistance and molecular epidemiology characteristics of methicillin-resistant Staphylococcus aureus in Shenzhen area

作　　者：李文青[1] 程锦娥[1] 吴伟元[1] 周小海[1] 卢月梅[1] 吴文苑[1] 龚文波 

机构地区：[1]暨南大学第二临床医学院/深圳市人民医院检验科/深圳市病原微生物及细菌耐药监控重点实验室,深圳518020

出　　处：《中国人兽共患病学报》2017年第12期1098-1102,1109,共6页Chinese Journal of Zoonoses

基　　金：深圳市知识创新计划基础研究项目(No.JCYJ20150403101146287)资助~~

摘　　要：目的调查深圳地区甲氧西林耐药金黄色葡萄球菌的耐药情况并了解其分子流行病学特征。方法收集2012年深圳地区3所医院的112株甲氧西林耐药金黄色葡萄球菌,琼脂稀释法测定对10种抗菌药物的最低抑菌浓度(minimum inhibitory concentration,MIC),聚合酶链反应(PCR)来检测杀白细胞毒素(Panton-Valentine leucocidin,PVL)基因,并对PVL基因阳性菌株行多位点基因序列分型(MLST)。结果 MRSA检出率26.2%,112株MRSA对复方新诺明、莫西沙星、环丙沙星、庆大霉素、红霉素、克林霉素和四环素的耐药率分别为4.46%、12.50%、16.96%、19.64%、46.42%、25.00%和26.79%,未发现万古霉素、利奈唑胺和替考拉宁耐药株;PVL阴性株和阳性株对10种抗菌药物的耐药率均无显著性差异;112株MRSA中,PVL基因阳性13株(11.61%),MLST分型为7种已知序列型和1种新的序列型,其中ST338和ST25最多。结论深圳地区MRSA检出率较低,PVL基因阳性率处于中等水平,具有明显不同于其他地区的独特的遗传背景。The aim of this study is to investigate antibiotic resistance and molecular epidemiology characteristics of methicillin-resistant Staphylococcus aureus(MRSA)in Shenzhen area.We collected 428 Staphylococcus aureus isolates from eight hospitals in Shenzhen in 2012.According to the results of minimum inhibitory concentration(MIC)to cefoxitin,26.2% of Staphylococcus aureus isolates(112/428)were identified as MRSA.The MIC of 10 antimicrobial agents was determined by agar dilution method.Panton-Valentine leucocidin(PVL)was detected by polymerase chain reaction(PCR).Positive strains of PVL were detected by multilocus sequence typing(MLST).Among the 112 strains,the resistance rates to trimethoprim-sulfamethoxazole,moxifloxacin,ciprofloxacin,gentamicin,erythromycin,clindamycin and tetracycline were 4.46%,12.50%,16.96%,19.64%,46.42%,25.00% and 26.79% respectively.No isolates resistant to vancomycin,linezolid and teicoplanin were found.Among the 112 strains,there were 13(11.61%)strains carried PVL gene.There were no significant differences in the resistance rates of PVL positive strains and negative strains to 10 kinds of antimicrobial agents.Among the 13 strains carried PVL gene,7 kinds of old sequences and 1 kind of new sequence type were found by MLST.ST338 and ST25 were the most common type.All the data indicate that surveillance of MRSA in Shenzhen has a distinct genetic background from other regions.

关 键 词：甲氧西林耐药金黄色葡萄球菌 琼脂稀释法 耐药性 杀白细胞毒素基因 多位点序列分型. 

分 类 号：R378.1[医药卫生—病原生物学]