2013年-2015年甘肃省平凉市5岁以下儿童病毒性腹泻监测结果分析  

Surveillance results analysis of viral diarrhea among children under 5 years old in Pingliang,Gansu during 2013-2015

作　　者：张亚孔 于德山[2] 何军霞[1] 刘丽[3] 阎峻[3] 

机构地区：[1]平凉市疾病预防控制中心检验科,甘肃平凉744000 [2]甘肃省疾病预防控制中心急性传染病防治科,甘肃兰州730000 [3]平凉市疾病预防控制中心急性传染病防治科,甘肃平凉744000

出　　处：《中国卫生检验杂志》2017年第23期3472-3474,3477,共4页Chinese Journal of Health Laboratory Technology

基　　金：甘肃及周边省区传染病病原谱流行规律研究(2012ZX-10004-208);平凉市传染病病原谱流行规律研究课题

摘　　要：目的调查平凉市5岁以下儿童病毒性腹泻4种常见病原流行病学特征。方法收集哨点医院5岁以下腹泻患儿粪便标本212份。采用酶联免疫吸咐测定法(ELISA)进行A组轮状病毒(RV)抗原检测,采用逆转录聚合酶链式反应(RT-PCR)对星状病毒(Ast V)、人杯状病毒(Hu CV)、肠道腺病毒(Ead V)及RV的基因型别检测。结果检出110例(51.9%)至少感染一种病毒。4种病毒检出率由高到低依次为Hu CV 21.7%、A-RV 17.0%、Ast V 17.0%、Ead V 9.0%。混合感染的病例数占25.5%(28/110),其中以Hu CV和Ast V重叠感染11份最为多见。以≤2岁散居儿童为主。冬季是诺如病毒发病高峰,以NV GⅡ为主;秋季是轮状病毒发病高峰,G9P8是主要流行株。结论 RV和Hu CV是引起婴幼儿病毒性腹泻的主要病原体,G9P8基因型RV和诺如病毒GⅡ型是主要流行株。Objective To investigate the etiological characteristics of four common pathogens in children under 5 years old with viral diarrhea in Pingliang. Methods 212 stool samples were collected from children under 5 years old with diarrhea. Group A rotavirus antigen was detected using enzyme-linked immunosorbent assay（ ELISA）; nucleic acid detection of astrovirus（ Ast V）,Human calicivirus（ Hu CV）,enteric adenovirus（ Ead V） and rotavirus genotying were conducted by reverse transcription-polymerase chain reaction（ RT-PCR）. Results Infection with at least one virus was detected in 110 samples（ 51. 9%）. Detection rate of the four viruses was 21. 7%（ Hu CV）,17. 0%（ Ast V）,17. 0%（ A-RV） and 9. 0%（ Ead V） in descending order. Children with mixed infection accounted for 25. 5%（ 28/110）,with mixed infection by Hu CV and Ast V as the most common（ n = 11）. Most cases were scattered at children aged ≤2 years old. The incidence peak period of Hu CV was winter,and NV GⅡwas the most common genotype. Autumn was the onset of the peak with rotavirus,and G9 P8 was the most common genotype. Conclusion The major pathogens of viral diarrhea in children were RV and Hu CV,and G9 P8 RV and NV GⅡ are the major epidemic strains.

关 键 词：婴幼儿腹泻 病毒 监测 流行病学 

分 类 号：R725.1[医药卫生—儿科]