乳酸菌和酵母菌混合发酵脱毒橡胶籽的安全性试验研究  

Experimental study on the safety of detoxified rubber seeds mixedly fermentated by lactic acid bacteria and yeast

作　　者：彭塘福 陈晗[1] 敬伟甫 林连兵[1] 邓先余[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]昆明益口福生物技术有限公司,云南昆明650500

出　　处：《扬州大学学报（农业与生命科学版）》2017年第3期62-67,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金应急管理项目(31640079)

摘　　要：选昆明小鼠130只,雌雄各半,随机分为13组,每组10只。7d灌胃急性毒性试验和30d灌胃慢性毒性试验各5组,30d饲喂试验3组,通过灌胃的给药方式测量每组小鼠的体重和脏器比,以验证融合魏斯菌(Weissella confusa)CMR-1与库德里阿兹威毕赤酵母(Pichia kudriavzevii)JM-2的安全性以及评估混合发酵脱毒橡胶籽的安全性。结果表明:在7d急性毒性试验及30d慢性毒性试验中,灌胃CMR-1、JM-2与对照组小鼠相比均无显著差异;在30d饲喂试验中,饲喂脱毒橡胶籽的小鼠与对照组相比无显著差异。饲喂未发酵脱毒橡胶籽的小鼠与对照组相比,心体比、肝体比出现极显著差异,脾体比、肺体比、肾体比无显著性差异。130 Kunming rats were randomly divided into 13 groups, and each group had ten rats. 5 groups were arranged in 7 d acute toxicity tests and 30 d chronic toxicity tests resepectively, and 3 groups were 30 d feeding tests. The body weight and organ ratio of each group mice were measured by garage administration. The objectives of the study were to verify the safety of Weissella confusa and Pichia kudriavzevii, and to evaluate the safety of detoxified rubber seeds which were mixedly fermentated. During the 7 d acute toxicity tests and 30 d chronic toxicity tests, the body weight and organ ratio of mice by feeding strain CMR-1, JM-2 compared with the MRS control group, YPD control group and normal control group were not significantly different （P〉0.05）. Organ ratio of mice that were fed detoxified rubber seeds com- pared with control groups showed no significant difference （P〉0.05） in the 30 d feeding tests. The heart and weight ratio, liver and weight ratio of mice which fed with no fermentated rubber seeds compared with that of control group, had a significant difference （P〈0.01）, and the spleen weight ratio, lung weight ratio and kidney weight ratio had no signifi- cant difference （P〉0.05）.

关 键 词：融合魏斯菌 库德里阿兹威毕赤酵母 橡胶籽 混合发酵 

分 类 号：S794.1[农业科学—林木遗传育种]