珠子参皂苷脂质体凝胶剂的制备及皮肤毒性实验研究  

Preparation of Saponin fromPanacis Majoris Rhizome Liposome Gel and Its Experimental Study of Skin Toxicity

作　　者：张继红[1] 王海燕[1] 李小妹 石孟琼[3] 李小琴 张永峰 陈茂华[1] 刘宏军[1] 罗涛[1] 邹坤 

机构地区：[1]三峡大学中医临床医学院/三峡大学脾胃病研究所,湖北宜昌443002 [2]三峡大学生物与制药学院,湖北宜昌443002 [3]三峡大学医学院,湖北宜昌443002

出　　处：《三峡大学学报（自然科学版）》2018年第1期100-105,108,共7页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金资助项目(81341141);湖北省宜昌市科技局资助项目(A15301-33);湖北省卫生计划生育委员会资助项目(201759)

摘　　要：研究珠子参皂苷脂质体及脂质体凝胶的制备方法,并观察其对动物皮肤毒性作用.采用超声-薄膜分散法制备珠子参皂苷脂质体,以卡波姆-940为基质制备脂质体凝胶;透析法测定珠子参皂苷脂质体以及脂质体凝胶中药物的释放,考察该制剂的皮肤毒性和稳定性.实验所得珠子参皂苷脂质体外观圆整,分布较均匀,其平均包封率为75.84±2.45;珠子参皂苷脂质体凝胶渗透速率显著高于珠子参皂苷凝胶的渗透速率;珠子参皂苷脂质体凝胶对兔完整和破损皮肤无明显急性毒性和刺激性作用,对豚鼠皮肤无过敏作用;不宜高温贮存,对光不稳定.结果表明珠子参皂苷脂质体凝胶剂体制备方法可行、简便、质量稳定、经皮给药安全,具有开发价值.The aim is to investigate the methods of preparation of saponin from Panacis majoris rhizome liposome and liposome gel, and observe its toxicity to animal skin. The saponin from Panacis majoris rhizome liposome is prepared by ultrasounic method and the carbomer-940 is added as a base for the preparation of lipo- some gel, and determined the liberation of drug by dialysis. The stability and skin toxicity are also studied. Our study results show that the obtained liposome has a sphere shape with narrow size distribution, its encap- sulation ratio is 75.84±2.45. The permeation rate of saponin from Panacis majoris rhizome liposome gel is sig- nificantly higher than that of Panacis majoris rhizome gel. Saponin from Panacis majoris rhizome liposome gel has no uuvious acute toxicity and irritation to the intact and damaged skin of rabbits, and has no allergic effect on the skin of guinea pigs. The preparation has unstable in the light and high temperature. This study results indicate that the preparation method is feasible, simple, stable in quality, and safe in transdermal drug delivery, which is valuable to be further investigated.

关 键 词：珠子参皂苷 脂质体 凝胶 质量评价 皮肤毒性 

分 类 号：R944[医药卫生—药剂学]